1 Koret School of Veterinary Medicine, The Hebrew University of Jerusalem , Rehovot, Israel .
2 INRA , UMR BIPAR INRA, Anses, ENVA 14 rue Pierre et Marie Curie, Maisons-Alfort, France .
Vector Borne Zoonotic Dis. 2017 Jan;17(1):42-50. doi: 10.1089/vbz.2016.1956.
Bartonellae are fastidious, facultative, intracellular vector-borne bacteria distributed among mammalian reservoirs worldwide. The pathogenic potential of many Bartonella spp. has increased the interest in these bacteria and advanced their research. Isolation of Bartonella spp. is laborious using classical bacteriological methods and requires specific conditions and prolonged incubation periods. In contrast, molecular methods for detection of Bartonella DNA are considered as more practical and sensitive than the former. Among the molecular methods, the use of real-time PCR assays for primary screening of Bartonella spp., followed by several molecular confirmatory assays, using either conventional or real-time PCR, is recommended. Although primary isolation of Bartonella is a laborious task, we encourage its application to all PCR-positive samples as this is the most reliable proof for the presence of live bacteria. Moreover, a successful trial will enable a broader molecular characterization and speciation of isolated colonies. The present guideline gathers and summarizes recommendations, including advantages and limitations of isolation and molecular detection of Bartonella from mammalian and arthropod samples.
巴尔通体是一种挑剔的、兼性的、细胞内的、分布于全球哺乳动物宿主中的、经媒介传播的细菌。许多巴尔通体物种的致病潜力增加了人们对这些细菌的兴趣，并推动了对它们的研究。使用经典的细菌学方法分离巴尔通体是很费力的，需要特定的条件和延长的孵育期。相比之下，检测巴尔通体 DNA 的分子方法被认为比前者更实用和敏感。在分子方法中，建议使用实时 PCR 检测作为初步筛选巴尔通体的方法，然后使用常规或实时 PCR 进行几种分子确认检测。虽然初步分离巴尔通体是一项费力的任务，但我们鼓励将其应用于所有 PCR 阳性样本，因为这是存在活菌的最可靠证据。此外，成功的试验将能够对分离的菌落进行更广泛的分子特征和分类。本指南收集和总结了从哺乳动物和节肢动物样本中分离和分子检测巴尔通体的建议，包括其优点和局限性。
