Sanz-González Silvia M, García-Medina José J, Zanón-Moreno Vicente, López-Gálvez María I, Galarreta-Mira David, Duarte Lilianne, Valero-Velló Mar, Ramírez Ana I, Arévalo J Fernando, Pinazo-Durán María D
Ophthalmic Research Unit "Santiago Grisolía", Fundación Investigación Sanitaria y Biomédica (FISABIO), Ave. Gaspar Aguilar 90, 46017 Valencia, Spain.
Cellular and Molecular Ophthalmo-Biology Group, University of Valencia, Ave. Blasco Ibañez 15, 46010 Valencia, Spain.
Antioxidants (Basel). 2020 Nov 9;9(11):1101. doi: 10.3390/antiox9111101.
Reactive oxygen species (ROS) overproduction and ROS-signaling pathways activation attack the eyes. We evaluated the oxidative stress (OS) and the effects of a daily, core nutritional supplement regimen containing antioxidants and omega 3 fatty acids (A/ω3) in type 2 diabetics (T2DM). A case-control study was carried out in 480 participants [287 T2DM patients with (+)/without (-) diabetic retinopathy (DR) and 193 healthy controls (CG)], randomly assigned to a daily pill of A/ω3. Periodic evaluation through 38 months allowed to outline patient characteristics, DR features, and classic/OS blood parameters. Statistics were performed by the SPSS 24.0 program. Diabetics displayed significantly higher circulating pro-oxidants ( = 0.001) and lower antioxidants ( = 0.0001) than the controls. Significantly higher plasma malondialdehyde/thiobarbituric acid reactive substances (MDA/TBARS; = 0.006) and lower plasma total antioxidant capacity (TAC; = 0.042) and vitamin C (0.020) was found in T2DM + DR versus T2DM-DR. The differential expression profile of solute carrier family 23 member 2 (SLC23A2) gene was seen in diabetics versus the CG ( = 0.001), and in T2DM + DR versus T2DM - DR ( < 0.05). The A/ω3 regime significantly reduced the pro-oxidants ( < 0.05) and augmented the antioxidants ( < 0.05). This follow-up study supports that a regular A/ω3 supplementation reduces the oxidative load and may serve as a dietary prophylaxis/adjunctive intervention for patients at risk of diabetic blindness.
活性氧（ROS）的过量产生和ROS信号通路的激活会损害眼睛。我们评估了2型糖尿病患者（T2DM）的氧化应激（OS）以及每日服用包含抗氧化剂和ω-3脂肪酸（A/ω3）的核心营养补充方案的效果。对480名参与者进行了一项病例对照研究[287名患有（+）/未患有（-）糖尿病视网膜病变（DR）的T2DM患者和193名健康对照者（CG）]，他们被随机分配每日服用一粒A/ω3药丸。经过38个月的定期评估，梳理出了患者特征、DR特征以及经典/OS血液参数。使用SPSS 24.0程序进行统计分析。与对照组相比，糖尿病患者的循环促氧化剂水平显著更高（ = 0.001），而抗氧化剂水平更低（ = 0.0001）。与T2DM-DR相比，T2DM + DR患者的血浆丙二醛/硫代巴比妥酸反应性物质（MDA/TBARS； = 0.006）显著更高，而血浆总抗氧化能力（TAC； = 0.042）和维生素C水平（0.020）更低。在糖尿病患者与CG之间（ = 0.001）以及T2DM + DR与T2DM - DR之间（ < 0.05）观察到溶质载体家族23成员2（SLC23A2）基因的差异表达谱。A/ω3方案显著降低了促氧化剂水平（ < 0.05）并提高了抗氧化剂水平（ < 0.05）。这项随访研究支持定期补充A/ω3可降低氧化负荷，并可作为糖尿病性失明风险患者的饮食预防/辅助干预措施。
