Imunologia Celular e Molecular, Instituto René Rachou, Belo Horizonte, MG 30190-002, Brazil.
Helmintologia e Malacologia Médica, Instituto René Rachou, Belo Horizonte, MG 30190-002, Brazil.
Viruses. 2020 Nov 10;12(11):1284. doi: 10.3390/v12111284.
Dengue is an acute viral disease caused by (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is palliative with acetaminophen (APAP), usually known as Paracetamol, being the most used drug aiming to relieve the mild symptoms of dengue. APAP is a safe and effective drug but, like dengue, can trigger the development of liver disorders. Given this scenario, it is necessary to investigate the effects of combining these two factors on hepatocyte homeostasis. Therefore, this study aimed to evaluate the molecular changes in hepatocytes resulting from the association between DENV infection and treatment with sub-toxic APAP concentrations. Using an in vitro experimental model of DENV-2 infected hepatocytes (AML-12 cells) treated with APAP, we evaluated the influence of the virus and drug association on the transcriptome of these hepatocytes by RNA sequencing (RNAseq). The virus-drug association was able to induce changes in the gene expression profile of AML-12 cells and here we highlight and explore these changes and its putative influence on biological processes for cellular homeostasis.
登革热是一种由 （DENV）引起的急性病毒病，被认为是全球最常见的虫媒病毒。登革热的临床特征可能从无症状到严重并发症和严重器官损伤不等，特别是对肝脏有影响。登革热的治疗方法是姑息性的，通常使用对乙酰氨基酚（APAP），即扑热息痛，作为缓解登革热轻度症状的最常用药物。APAP 是一种安全有效的药物，但与登革热一样，也可能引发肝脏疾病。鉴于这种情况，有必要研究这两个因素结合对肝细胞内稳态的影响。因此，本研究旨在评估 DENV 感染和亚毒性 APAP 浓度治疗联合对肝细胞的分子变化。我们使用 DENV-2 感染的肝细胞（AML-12 细胞）的体外实验模型，通过 RNA 测序（RNAseq）评估病毒和药物联合对这些肝细胞转录组的影响。病毒-药物联合能够诱导 AML-12 细胞的基因表达谱发生变化，在这里我们强调并探讨了这些变化及其对细胞内稳态生物学过程的可能影响。
