• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

放线菌分离物抗群体感应和抗生物膜活性的筛选和定量及其对食品腐败生物膜形成菌的抑制作用。

Screening and quantification of anti-quorum sensing and antibiofilm activity of Actinomycetes isolates against food spoilage biofilm-forming bacteria.

机构信息

Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Raya Cisauk-Lapan No. 10, Sampora, Cisauk, Tangerang, Banten, 15345, Indonesia.

出版信息

BMC Microbiol. 2021 Jan 2;21(1):1. doi: 10.1186/s12866-020-02060-7.

DOI:10.1186/s12866-020-02060-7
PMID:33386072
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7777393/
Abstract

BACKGROUND

Biofilms can form in many industries, one of them is the food industry. The formation of biofilms in this industry could cause immense economic losses and endanger public health. Biofilms formation is mainly triggered by quorum sensing. Therefore, inhibition of quorum sensing could be an innovative approach to inhibit the formation of biofilms. One way to inhibit quorum sensing is by using anti-quorum sensing compounds. Actinomycetes are a group of bacteria that is acknowledged to produce these compounds.

RESULTS

There were eight crude extracts of Actinomycetes isolates that showed promising anti-quorum sensing activity against Chromobacterium violaceum. The concentration of the crude extracts was 20 mg/mL. All the crude extracts showed no antibacterial activity against food spoilage bacteria, except for crude extracts of isolate 18 PM that showed antibacterial activity against Bacillus subtilis. They also showed various antibiofilm activity, both inhibition and destruction. The highest inhibition and destruction activity sequentially was done by crude extracts of isolate 12 AC with 89.60% against Bacillus cereus and crude extracts of isolate SW03 with 93.06% against Shewanella putrefaciens.

CONCLUSIONS

Actinomycetes isolates that isolated from different regions in Indonesia can be used as potential candidates to overcome biofilms formed by food spoilage bacteria using their ability to produce anti-quorum sensing compounds.

摘要

背景

生物膜可以在许多行业中形成，其中之一是食品行业。该行业中生物膜的形成可能会造成巨大的经济损失，并危及公众健康。生物膜的形成主要是由群体感应触发的。因此，抑制群体感应可能是抑制生物膜形成的一种创新方法。抑制群体感应的一种方法是使用抗群体感应化合物。放线菌是一类被公认为能产生这些化合物的细菌。

结果

有 8 种放线菌分离株的粗提物对紫色杆菌表现出有希望的抗群体感应活性。粗提物的浓度为 20mg/ml。除了分离株 18PM 的粗提物对枯草芽孢杆菌表现出抗菌活性外，所有粗提物对食品腐败菌均无抗菌活性。它们还表现出不同的抗生物膜活性，包括抑制和破坏。抑制和破坏活性最高的依次是分离株 12AC 的粗提物，对蜡状芽孢杆菌的抑制率为 89.60%，以及分离株 SW03 的粗提物，对腐败希瓦氏菌的抑制率为 93.06%。

结论

从印度尼西亚不同地区分离得到的放线菌分离株可以作为潜在的候选物，利用其产生抗群体感应化合物的能力来克服由食品腐败菌形成的生物膜。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1918/7777393/4cb93ec408f7/12866_2020_2060_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1918/7777393/f36409ed4b5e/12866_2020_2060_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1918/7777393/e48b9e7af13a/12866_2020_2060_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1918/7777393/4cb93ec408f7/12866_2020_2060_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1918/7777393/f36409ed4b5e/12866_2020_2060_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1918/7777393/e48b9e7af13a/12866_2020_2060_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1918/7777393/4cb93ec408f7/12866_2020_2060_Fig3_HTML.jpg

相似文献

1
Screening and quantification of anti-quorum sensing and antibiofilm activity of Actinomycetes isolates against food spoilage biofilm-forming bacteria.放线菌分离物抗群体感应和抗生物膜活性的筛选和定量及其对食品腐败生物膜形成菌的抑制作用。
BMC Microbiol. 2021 Jan 2;21(1):1. doi: 10.1186/s12866-020-02060-7.
2
Screening of antibiofilm and anti-quorum sensing activty of Actinomycetes isolates extracts against aquaculture pathogenic bacteria.放线菌分离物提取物对水产养殖病原菌的抗生物膜和抗群体感应活性的筛选。
BMC Microbiol. 2020 Nov 12;20(1):343. doi: 10.1186/s12866-020-02022-z.
3
Control of pathogenic bacteria using marine actinobacterial extract with antiquorum sensing and antibiofilm activity.利用具有群体感应抑制和抗生物膜活性的海洋放线菌提取物控制病原菌。
BMC Res Notes. 2023 Nov 2;16(1):305. doi: 10.1186/s13104-023-06580-z.
4
Screening and quantification of anti-quorum sensing and antibiofilm activities of phyllosphere bacteria against biofilm forming bacteria.叶际细菌对生物膜形成细菌的群体感应抑制和抗生物膜活性的筛选与定量分析
BMC Res Notes. 2019 Nov 7;12(1):732. doi: 10.1186/s13104-019-4775-1.
5
The natural antimicrobial carvacrol inhibits quorum sensing in Chromobacterium violaceum and reduces bacterial biofilm formation at sub-lethal concentrations.天然抗菌剂香芹酚可抑制紫色色杆菌中的群体感应，并在亚致死浓度下减少细菌生物膜的形成。
PLoS One. 2014 Apr 1;9(4):e93414. doi: 10.1371/journal.pone.0093414. eCollection 2014.
6
Citral and its derivatives inhibit quorum sensing and biofilm formation in Chromobacterium violaceum.柠檬醛及其衍生物可抑制紫色色杆菌中的群体感应和生物膜形成。
Arch Microbiol. 2021 May;203(4):1451-1459. doi: 10.1007/s00203-020-02127-z. Epub 2021 Jan 3.
7
Anti-biofilm forming and anti-quorum sensing activity of selected essential oils and their main components on food-related micro-organisms.某些精油及其主要成分对食源相关微生物的抗生物膜形成和抗群体感应活性。
J Appl Microbiol. 2013 Oct;115(4):933-42. doi: 10.1111/jam.12289. Epub 2013 Jul 10.
8
Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria.从叶际细菌中提取具有抗生物膜和群体感应淬灭活性的物质，以控制多种鱼类病原菌。
BMC Res Notes. 2021 May 25;14(1):202. doi: 10.1186/s13104-021-05612-w.
9
Anti-quorum Sensing and Anti-biofilm Activity of Extract by Attenuating the Quorum Sensing-Controlled Virulence Factor Production in .提取物通过减弱群体感应控制的毒力因子产生的抗群体感应和抗生物膜活性
Front Cell Infect Microbiol. 2017 Jul 26;7:337. doi: 10.3389/fcimb.2017.00337. eCollection 2017.
10
Antimicrobial peptide AMPNT-6 from Bacillus subtilis inhibits biofilm formation by Shewanella putrefaciens and disrupts its preformed biofilms on both abiotic and shrimp shell surfaces.枯草芽孢杆菌来源的抗菌肽 AMPNT-6 抑制腐生希瓦氏菌的生物膜形成，并破坏其在非生物和虾壳表面形成的预生物膜。
Food Res Int. 2017 Dec;102:8-13. doi: 10.1016/j.foodres.2017.09.057. Epub 2017 Sep 30.

引用本文的文献

1
Control of biofilm from single and multispecies bacteria associated with food spoilage using metabolite of Streptomyces sp. KP110 and Pseudomonas fluorescens JB 3B.利用链霉菌属KP110和荧光假单胞菌JB 3B的代谢产物控制与食品腐败相关的单菌种和多菌种细菌生物膜
Sci Rep. 2025 Jul 4;15(1):23956. doi: 10.1038/s41598-025-09259-6.
2
Skin-associated commensal microorganisms and their metabolites.皮肤共生微生物及其代谢产物。
J Appl Microbiol. 2025 May 2;136(5). doi: 10.1093/jambio/lxaf111.
3
A comprehensive One Health investigation of erythromycin and quinupristin/dalfopristin resistant spp. in Iran.

本文引用的文献

1
Antibiofilm effect of Nocardiopsis sp. GRG 1 (KT235640) compound against biofilm forming Gram negative bacteria on UTIs.格氏糖多孢菌（GRG1，KT235640）化合物对尿路感染中生物膜形成革兰氏阴性菌的抗生物膜作用。
Microb Pathog. 2018 May;118:190-198. doi: 10.1016/j.micpath.2018.03.011. Epub 2018 Mar 7.
2
Actinomycetes benefaction role in soil and plant health.放线菌在土壤和植物健康中的有益作用。
Microb Pathog. 2017 Oct;111:458-467. doi: 10.1016/j.micpath.2017.09.036. Epub 2017 Sep 18.
3
Anti-Quorum Sensing Activity of Substances Isolated from Wild Berry Associated Bacteria.
对伊朗耐红霉素和奎奴普丁/达福普汀的[具体物种]进行的全面“同一健康”调查。 （注：原文中spp.未明确写出具体物种，所以翻译时保留英文）
J Water Health. 2025 Apr;23(4):439-449. doi: 10.2166/wh.2025.174. Epub 2025 Apr 8.
4
Bioactive Fraction of Streptomyces thinghirensis MSA1 Effectively Inhibits Biofilm Forming Clinically Significant AMR Pathogens.廷希尔链霉菌MSA1的生物活性组分有效抑制形成生物膜的临床重要耐药病原体。
Curr Microbiol. 2025 Apr 6;82(6):234. doi: 10.1007/s00284-025-04214-0.
5
Temporal and Depth-Driven Variability of Pelagic Bacterial Communities in Lake Erie: Biofilm and Plankton Dynamics.伊利湖浮游细菌群落的时间和深度驱动变异性：生物膜与浮游生物动态
Environ Microbiol Rep. 2025 Apr;17(2):e70079. doi: 10.1111/1758-2229.70079.
6
Assessment of antibiofilm and quorum quenching potencies of environmental bacteria in controlling biofilm of food spoilage bacteria.评估环境细菌在控制食品腐败细菌生物膜方面的抗生物膜和群体感应淬灭能力。
BMC Res Notes. 2025 Feb 17;18(1):71. doi: 10.1186/s13104-025-07141-2.
7
Diabetes and gut microbiome.糖尿病与肠道微生物群
Front Microbiol. 2025 Jan 7;15:1451054. doi: 10.3389/fmicb.2024.1451054. eCollection 2024.
8
Prevalence and genomic insights of carbapenem resistant and ESBL producing Multidrug resistant Escherichia coli in urinary tract infections.泌尿系统感染中耐碳青霉烯类及产超广谱β-内酰胺酶的多重耐药大肠杆菌的流行情况及基因组分析
Sci Rep. 2025 Jan 20;15(1):2541. doi: 10.1038/s41598-024-84754-w.
9
Antimicrobial resistance in fish and poultry: Public health implications for animal source food production in Nigeria, Egypt, and South Africa.鱼类和家禽中的抗菌药物耐药性：对尼日利亚、埃及和南非动物源食品生产的公共卫生影响
Front Antibiot. 2022 Nov 10;1:1043302. doi: 10.3389/frabi.2022.1043302. eCollection 2022.
10
The synergistic interaction of systemic inflammation, dysbiosis and antimicrobial resistance promotes growth restriction in children with acute severe malnutrition: An emphasis on .全身炎症、生态失调和抗菌药物耐药性的协同相互作用促进了急性重度营养不良儿童的生长受限：重点关注于…… （原文此处不完整）
Front Antibiot. 2022 Oct 24;1:1001717. doi: 10.3389/frabi.2022.1001717. eCollection 2022.
从野生浆果相关细菌中分离出的物质的群体感应抑制活性
Avicenna J Med Biotechnol. 2017 Jan-Mar;9(1):23-30.
4
Bacillus cereus Biofilms-Same, Only Different.蜡样芽孢杆菌生物膜——相同却又不同
Front Microbiol. 2016 Jul 7;7:1054. doi: 10.3389/fmicb.2016.01054. eCollection 2016.
5
Secondary metabolites produced by marine streptomyces as antibiofilm and quorum-sensing inhibitor of uropathogen Proteus mirabilis.海洋链霉菌产生的次生代谢产物作为尿路致病性奇异变形杆菌的抗生物膜和群体感应抑制剂。
Environ Sci Pollut Res Int. 2016 Mar;23(5):4756-67. doi: 10.1007/s11356-015-5687-9. Epub 2015 Nov 4.
6
Quorum sensing inhibitors as anti-biofilm agents.群体感应抑制剂作为抗生物膜剂
Curr Pharm Des. 2015;21(1):5-11. doi: 10.2174/1381612820666140905114627.
7
Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection.群体感应淬灭酶及其在降解信号分子以阻断群体感应依赖性感染中的应用。
Int J Mol Sci. 2013 Aug 26;14(9):17477-500. doi: 10.3390/ijms140917477.
8
Biologically inspired strategies for combating bacterial biofilms.受生物启发的抗细菌生物膜策略。
Curr Opin Pharmacol. 2013 Oct;13(5):699-706. doi: 10.1016/j.coph.2013.07.004. Epub 2013 Jul 18.
9
Anti-biofilm forming and anti-quorum sensing activity of selected essential oils and their main components on food-related micro-organisms.某些精油及其主要成分对食源相关微生物的抗生物膜形成和抗群体感应活性。
J Appl Microbiol. 2013 Oct;115(4):933-42. doi: 10.1111/jam.12289. Epub 2013 Jul 10.
10
Quorum sensing and phytochemicals.群体感应与植物化学物质。
Int J Mol Sci. 2013 Jun 17;14(6):12607-19. doi: 10.3390/ijms140612607.