电针对东莨菪碱诱导的记忆障碍的改善作用可能与调控 miR-210/SIN3A 及 miR-183/SIN3A 信号通路有关。
Electroacupuncture reduces scopolamine-induced amnesia via mediating the miR-210/SIN3A and miR-183/SIN3A signaling pathway.
机构信息
Department of Anesthesiology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, No.136, Jingzhou Street, Xiangcheng District, Xiangyang, 441021, Hubei, China.
出版信息
Mol Med. 2020 Nov 12;26(1):107. doi: 10.1186/s10020-020-00233-8.
BACKGROUND
The expression of SIN3A is closely correlated with electroacupuncture (EA) treatment efficacy of scopolamine-induced amnesia (SIA), but its underlying mechanisms remain to be further explored.
METHODS
Quantitative real-time PCR was performed to analyze the expression of candidate microRNAs (miRNAs) and SIN3A mRNA in a rat model of SIA. Western blot was carried out to evaluate the differential expression of SIN3A proteins under different circumstances. Luciferase assay was used to explore the inhibitory role of certain miRNAs in SIN3A expression. A novel object recognition (NOR) test was performed to assess the memory function of SIA rats undergoing EA treatment. Immunohistochemistry was carried out to evaluate the expression of SIN3A in the hippocampus of SIA rats.
RESULTS
Rno-miR-183-5p, rno-miR-34c-3p and rno-miR-210-3p were significantly up-regulated in SIA rats treated with EA. In addition, rno-miR-183-5p and rno-miR-210-3p exerted an inhibitory effect on SIN3A expression. EA treatment of SIA rats effectively restored the dysregulated expression of rno-miR-183-5p, rno-miR-210-3p and SIN3A. EA treatment also promoted the inhibited expression of neuronal IEGs including Arc, Egr1, Homer1 and Narp in the hippocampus of SIA rats. Accordingly, the NOR test also confirmed the effect of EA treatment on the improvement of memory in SIA rats.
CONCLUSION
In summary, the findings of this study demonstrated that scopolamine-induced amnesia was associated with downregulated expression of miR-210/miR-183 and upregulated expression of SIN3A. Furthermore, treatment with EA alleviated scopolamine-induced amnesia in rats and was associated with upregulated expression of miR-210/miR-183 and downregulated expression of SIN3A.
背景
SIN3A 的表达与东莨菪碱诱导的记忆障碍（SIA）的电针（EA）治疗效果密切相关，但具体机制仍需进一步探索。
方法
采用实时定量 PCR 分析 SIA 大鼠模型中候选 microRNAs（miRNAs）和 SIN3A mRNA 的表达。Western blot 评估不同情况下 SIN3A 蛋白的差异表达。采用荧光素酶报告基因实验探讨特定 miRNAs 对 SIN3A 表达的抑制作用。通过 novel object recognition（NOR）测试评估 SIA 大鼠接受 EA 治疗后的记忆功能。免疫组织化学评估 SIA 大鼠海马中 SIN3A 的表达。
结果
EA 治疗 SIA 大鼠后，rno-miR-183-5p、rno-miR-34c-3p 和 rno-miR-210-3p 表达明显上调。此外，rno-miR-183-5p 和 rno-miR-210-3p 对 SIN3A 表达具有抑制作用。EA 治疗 SIA 大鼠有效恢复了 rno-miR-183-5p、rno-miR-210-3p 和 SIN3A 的失调表达。EA 治疗还促进了 SIA 大鼠海马中神经元 IEGs（包括 Arc、Egr1、Homer1 和 Narp）的抑制表达。因此，NOR 测试也证实了 EA 治疗对 SIA 大鼠记忆改善的作用。
结论
综上所述，本研究结果表明，东莨菪碱诱导的记忆障碍与 miR-210/miR-183 表达下调和 SIN3A 表达上调有关。此外，EA 治疗缓解了 SIA 大鼠的记忆障碍，与 miR-210/miR-183 表达上调和 SIN3A 表达下调有关。
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