• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

动态原子力显微镜检测低密度脂蛋白在氧化诱导下大小、硬度和粘性的变化。

Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein.

机构信息

Jiangxi Key Laboratory for Microscale interdisciplinary Study, Institute for Advanced Study, Nanchang University, Nanchang, 330031, Jiangxi, P. R. China.

School of Materials Science and Engineering, Nanchang University, 999 Xuefu Ave., Honggutan District, Nanchang, 330031, Jiangxi, P. R. China.

出版信息

J Nanobiotechnology. 2020 Nov 12;18(1):167. doi: 10.1186/s12951-020-00727-x.

DOI:10.1186/s12951-020-00727-x
PMID:33183326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7664080/
Abstract

BACKGROUND

Low-density lipoprotein (LDL) is an important plasma lipoprotein transporting lipids to peripheral tissues/cells. The oxidation of LDL plays critical roles in atherogenesis and its oxidized form (oxLDL) is an important risk factor of atherosclerosis. The biomechanical properties of LDL/oxLDL are closely correlated with the disease. To date, however, the oxidation-induced changes in size and biomechanical properties (stiffness and stickiness) of LDL particles are less investigated.

METHODS

In this study, copper-induced LDL oxidation was confirmed by detecting electrophoretic mobility, malondialdehyde production, and conjugated diene formation. Then, the topographical and biomechanical mappings of LDL particles before/after and during oxidation were performed by using atomic force microscopy (AFM) and the size and biomechanical forces of particles were measured and quantitatively analyzed.

RESULTS

Oxidation induced a significant decrease in size and stiffness (Young's modulus) but a significant increase in stickiness (adhesion force) of LDL particles. The smaller, softer, and stickier characteristics of oxidized LDL (oxLDL) partially explains its pro-atherosclerotic role.

CONCLUSIONS

The data implies that LDL oxidation probably aggravates atherogenesis by changing the size and biomechanical properties of LDL particles. The data may provide important information for a better understanding of LDL/oxLDL and atherosclerosis.

摘要

背景

低密度脂蛋白（LDL）是一种重要的血浆脂蛋白，负责将脂质转运至外周组织/细胞。LDL 的氧化在动脉粥样硬化形成中起着关键作用，其氧化形式（oxLDL）是动脉粥样硬化的一个重要危险因素。LDL/oxLDL 的生物力学特性与该疾病密切相关。然而，迄今为止，LDL 颗粒的氧化诱导的大小和生物力学特性（刚性和粘性）变化研究较少。

方法

在这项研究中，通过检测电泳迁移率、丙二醛产生和共轭二烯形成来确认铜诱导的 LDL 氧化。然后，通过原子力显微镜（AFM）对 LDL 颗粒在氧化前后及氧化过程中的形貌和生物力学进行映射，并测量和定量分析颗粒的大小和生物力学力。

结果

氧化导致 LDL 颗粒的大小和刚性（杨氏模量）显著降低，但粘性（粘附力）显著增加。氧化 LDL（oxLDL）的更小、更软、更粘性的特征部分解释了其促动脉粥样硬化的作用。

结论

这些数据表明，LDL 氧化可能通过改变 LDL 颗粒的大小和生物力学特性来加重动脉粥样硬化形成。这些数据可能为更好地理解 LDL/oxLDL 和动脉粥样硬化提供重要信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/dab18bb7b6ec/12951_2020_727_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/a9ed5a933949/12951_2020_727_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/c67f1f46ef79/12951_2020_727_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/bc107c8832fa/12951_2020_727_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/dab18bb7b6ec/12951_2020_727_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/a9ed5a933949/12951_2020_727_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/c67f1f46ef79/12951_2020_727_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/bc107c8832fa/12951_2020_727_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c4d/7664080/dab18bb7b6ec/12951_2020_727_Fig4_HTML.jpg

相似文献

1
Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein.动态原子力显微镜检测低密度脂蛋白在氧化诱导下大小、硬度和粘性的变化。
J Nanobiotechnology. 2020 Nov 12;18(1):167. doi: 10.1186/s12951-020-00727-x.
2
AFM detects the effects of acidic condition on the size and biomechanical properties of native/oxidized low-density lipoprotein.原子力显微镜检测酸性条件对天然/氧化低密度脂蛋白的大小和生物力学特性的影响。
Colloids Surf B Biointerfaces. 2021 Dec;208:112053. doi: 10.1016/j.colsurfb.2021.112053. Epub 2021 Aug 19.
3
Elastic modulus of low-density lipoprotein as potential indicator of its oxidation.低密度脂蛋白的弹性模量作为其氧化的潜在指标。
Ann Clin Biochem. 2015 Nov;52(Pt 6):647-53. doi: 10.1177/0004563215584958. Epub 2015 Apr 17.
4
Exogenous GM3 ganglioside inhibits atherosclerosis via multiple steps: A potential atheroprotective drug.外源性 GM3 神经节苷脂通过多个步骤抑制动脉粥样硬化：一种有潜力的动脉粥样硬化保护药物。
Pharmacol Res. 2019 Oct;148:104445. doi: 10.1016/j.phrs.2019.104445. Epub 2019 Sep 14.
5
Effects of cyclodextrins on the structure of LDL and its susceptibility to copper-induced oxidation.环糊精对低密度脂蛋白结构及其对铜诱导氧化的敏感性的影响。
Eur J Pharm Sci. 2016 Aug 25;91:183-9. doi: 10.1016/j.ejps.2016.04.037. Epub 2016 Apr 30.
6
Measurement of single low-density lipoprotein particles by atomic force microscopy.原子力显微镜法测量单低密度脂蛋白颗粒。
Ann Clin Biochem. 2013 Nov;50(Pt 6):564-70. doi: 10.1177/0004563213481586. Epub 2013 Jul 30.
7
Characterization of peroxynitrite-oxidized low density lipoprotein binding to human CD36.过氧亚硝酸盐氧化的低密度脂蛋白与人CD36结合的特性研究
Atherosclerosis. 2001 Mar;155(1):19-28. doi: 10.1016/s0021-9150(00)00524-4.
8
Imaging and force measurement of LDL and HDL by AFM in air and liquid.利用原子力显微镜在空气和液体环境中对低密度脂蛋白（LDL）和高密度脂蛋白（HDL）进行成像及力测量。
FEBS Open Bio. 2015 Apr 7;5:276-82. doi: 10.1016/j.fob.2015.03.014. eCollection 2015.
9
Adsorption of low-density lipoprotein, its oxidation, and subsequent binding of specific recombinant antibodies: An in situ ellipsometric study.低密度脂蛋白的吸附、其氧化以及特定重组抗体的后续结合：一项原位椭偏测量研究。
Biochim Biophys Acta. 2011 Feb;1810(2):211-7. doi: 10.1016/j.bbagen.2010.10.006. Epub 2010 Oct 20.
10
Comparative investigation on the sizes and scavenger receptor binding of human native and modified lipoprotein particles with atomic force microscopy.原子力显微镜比较研究人天然和修饰脂蛋白颗粒的大小和清道夫受体结合。
J Nanobiotechnology. 2018 Mar 21;16(1):25. doi: 10.1186/s12951-018-0352-3.

引用本文的文献

1
Copper Homeostasis and Cuproptosis As Potential Intervention Strategy in Atherosclerosis.铜稳态与铜死亡作为动脉粥样硬化潜在干预策略
J Cardiovasc Transl Res. 2025 Jul 21. doi: 10.1007/s12265-025-10661-8.
2
AFM reveals differential effects of acidification on LDL- and oxidized LDL-receptor interactions: biomechanical implications in atherogenesis.原子力显微镜揭示酸化对低密度脂蛋白及氧化低密度脂蛋白受体相互作用的不同影响：动脉粥样硬化形成中的生物力学意义
Cell Mol Biol Lett. 2025 Mar 18;30(1):32. doi: 10.1186/s11658-025-00715-9.
3
The role of metabolites in the progression of osteoarthritis: Mechanisms and advances in therapy.

本文引用的文献

1
Mapping the surface potential, charge density and adhesion of cellulose nanocrystals using advanced scanning probe microscopy.利用先进的扫描探针显微镜绘制纤维素纳米晶体的表面电势、电荷密度和粘附力。
Carbohydr Polym. 2020 Oct 15;246:116393. doi: 10.1016/j.carbpol.2020.116393. Epub 2020 May 4.
2
Can atherosclerosis be cured?动脉粥样硬化可以治愈吗？
Curr Opin Lipidol. 2019 Dec;30(6):477-484. doi: 10.1097/MOL.0000000000000644.
3
Exogenous GM3 ganglioside inhibits atherosclerosis via multiple steps: A potential atheroprotective drug.
代谢物在骨关节炎进展中的作用：治疗机制与进展
J Orthop Translat. 2025 Jan 7;50:56-70. doi: 10.1016/j.jot.2024.10.003. eCollection 2025 Jan.
4
Lipoprotein particles exhibit distinct mechanical properties.脂蛋白颗粒具有独特的机械特性。
J Extracell Biol. 2022 Dec 18;1(12):e68. doi: 10.1002/jex2.68. eCollection 2022 Dec.
5
Biomechanics-mediated endocytosis in atherosclerosis.动脉粥样硬化中生物力学介导的内吞作用
Front Cardiovasc Med. 2024 Apr 4;11:1337679. doi: 10.3389/fcvm.2024.1337679. eCollection 2024.
6
Lipoprotein Particles as Shuttles for Hydrophilic Cargo.作为亲水性货物载体的脂蛋白颗粒
Membranes (Basel). 2023 Apr 28;13(5):471. doi: 10.3390/membranes13050471.
7
Atomic force microscopy reveals involvement of the cell envelope in biomechanical properties of sickle erythrocytes.原子力显微镜揭示了细胞包膜在镰状红细胞生物力学特性中的作用。
BMC Biol. 2023 Feb 13;21(1):31. doi: 10.1186/s12915-023-01523-3.
8
Ligustrazine as an Extract from Medicinal and Edible Plant Chuanxiong Encapsulated in Liposome-Hydrogel Exerting Antioxidant Effect on Preventing Skin Photoaging.川芎中提取的川芎嗪包封于脂质体-水凝胶中对预防皮肤光老化具有抗氧化作用。
Polymers (Basel). 2022 Nov 7;14(21):4778. doi: 10.3390/polym14214778.
9
NLRP3 Inflammasome Activation Controls Vascular Smooth Muscle Cells Phenotypic Switch in Atherosclerosis.NLRP3 炎性小体激活调控动脉粥样硬化中血管平滑肌细胞表型转换。
Int J Mol Sci. 2021 Dec 29;23(1):340. doi: 10.3390/ijms23010340.
外源性 GM3 神经节苷脂通过多个步骤抑制动脉粥样硬化：一种有潜力的动脉粥样硬化保护药物。
Pharmacol Res. 2019 Oct;148:104445. doi: 10.1016/j.phrs.2019.104445. Epub 2019 Sep 14.
4
AFM assessing of nanomechanical fingerprints for cancer early diagnosis and classification: from single cell to tissue level.原子力显微镜（AFM）评估纳米力学指纹图谱在癌症早期诊断和分类中的应用：从单细胞到组织水平。
Nanoscale. 2018 Dec 7;10(45):20930-20945. doi: 10.1039/c8nr06146g. Epub 2018 Nov 8.
5
Effects of enzymes on elastic modulus of low-density lipoproteins were investigated using atomic force microscopy.利用原子力显微镜研究了酶对低密度脂蛋白弹性模量的影响。
Biochem Biophys Res Commun. 2018 Jun 27;501(3):607-611. doi: 10.1016/j.bbrc.2018.04.211.
6
Comparative investigation on the sizes and scavenger receptor binding of human native and modified lipoprotein particles with atomic force microscopy.原子力显微镜比较研究人天然和修饰脂蛋白颗粒的大小和清道夫受体结合。
J Nanobiotechnology. 2018 Mar 21;16(1):25. doi: 10.1186/s12951-018-0352-3.
7
Role of dual lipid-lowering therapy in coronary atherosclerosis regression: Evidence from recent studies.双重降脂治疗在冠状动脉粥样硬化消退中的作用：来自近期研究的证据。
Atherosclerosis. 2018 Feb;269:219-228. doi: 10.1016/j.atherosclerosis.2018.01.012. Epub 2018 Jan 17.
8
AFM contribution to unveil pro- and eukaryotic cell mechanical properties.原子力显微镜在揭示原核和真核细胞力学特性方面的贡献。
Semin Cell Dev Biol. 2018 Jan;73:177-187. doi: 10.1016/j.semcdb.2017.08.032. Epub 2017 Aug 19.
9
In situ AFM imaging of apolipoprotein A-I directly derived from plasma HDL.直接源自血浆高密度脂蛋白的载脂蛋白A-I的原位原子力显微镜成像。
Atherosclerosis. 2017 Apr;259:5-11. doi: 10.1016/j.atherosclerosis.2017.02.022. Epub 2017 Feb 24.
10
Effects of cyclodextrins on the structure of LDL and its susceptibility to copper-induced oxidation.环糊精对低密度脂蛋白结构及其对铜诱导氧化的敏感性的影响。
Eur J Pharm Sci. 2016 Aug 25;91:183-9. doi: 10.1016/j.ejps.2016.04.037. Epub 2016 Apr 30.