Weber Florian, Axmann Markus, Horner Andreas, Schwarzinger Bettina, Weghuber Julian, Plochberger Birgit
Department of Medical Engineering, University of Applied Sciences Upper Austria, 4020 Linz, Austria.
Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17164 Solna, Sweden.
Membranes (Basel). 2023 Apr 28;13(5):471. doi: 10.3390/membranes13050471.
Lipoprotein particles (LPs) are excellent transporters and have been intensively studied in cardiovascular diseases, especially regarding parameters such as their class distribution and accumulation, site-specific delivery, cellular internalization, and escape from endo/lysosomal compartments. The aim of the present work is the hydrophilic cargo loading of LPs. As an exemplary proof-of-principle showcase, the glucose metabolism-regulating hormone, insulin, was successfully incorporated into high-density lipoprotein (HDL) particles. The incorporation was studied and verified to be successful using Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM). Single-molecule-sensitive FM together with confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).
脂蛋白颗粒（LPs）是出色的转运体，并且在心血管疾病方面已得到深入研究，特别是在诸如它们的类别分布与积累、位点特异性递送、细胞内化以及从内体/溶酶体区室逃逸等参数方面。本研究的目的是实现脂蛋白颗粒的亲水性货物装载。作为一个示例性的原理验证展示，调节葡萄糖代谢的激素胰岛素成功地被整合到高密度脂蛋白（HDL）颗粒中。使用原子力显微镜（AFM）和荧光显微镜（FM）对这种整合进行了研究并证实是成功的。单分子敏感型FM与共聚焦成像一起可视化了单个载有胰岛素的HDL颗粒的膜相互作用以及随后葡萄糖转运蛋白4（Glut4）的细胞转运。
