Department of Materials Science and Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea; Center for Multidimensional Programmable Matter, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea.
Department of Materials Science and Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea; Center for Multidimensional Programmable Matter, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea.
Carbohydr Polym. 2021 Jan 15;252:117128. doi: 10.1016/j.carbpol.2020.117128. Epub 2020 Sep 22.
Alginate is an abundant natural polysaccharide widely utilized in various biomedical applications. Alginate also possesses numerous hydroxyl and carboxylate functional groups that allow chemical modifications to introduce different functionalities. However, it is difficult to apply various chemical reactions to alginate due to limited solubility in organic solvents. Herein, functional moieties for radical polymerization and cell adhesion were separately conjugated to hydroxyl and carboxylate groups of alginate, respectively, in order to independently control the crosslinking density and cell adhesive properties of hydrogels. Sodium counterions of alginate are first substituted with tetrabutylammonium ions to facilitate the dissolution in an organic solvent, followed by in situ conjugations of (1) cell adhesion molecules (CAM) via carbodiimide-mediated amide formation and (2) methacrylate via ring-opening nucleophilic reaction. The resulting CAM-linked methacrylic alginate was able to not only crosslink different monomers to form hydrogels with varying mechanical properties, but also induce stable cell adhesion to the hydrogels.
海藻酸盐是一种丰富的天然多糖，广泛应用于各种生物医学应用中。海藻酸盐还具有许多羟基和羧基官能团，允许进行化学修饰以引入不同的功能。然而，由于在有机溶剂中的溶解度有限，很难将各种化学反应应用于海藻酸盐。在此，自由基聚合和细胞黏附的功能基部分分别与海藻酸盐的羟基和羧基连接，以分别独立控制水凝胶的交联密度和细胞黏附特性。海藻酸盐的钠离子首先被四丁基铵离子取代，以促进其在有机溶剂中的溶解，然后通过（1）细胞黏附分子（CAM）的碳二亚胺介导的酰胺形成和（2）甲基丙烯酸酯的开环亲核反应进行原位共轭。所得的 CAM 连接的甲基丙烯酰化海藻酸盐不仅能够交联不同的单体形成具有不同机械性能的水凝胶，而且还能够诱导细胞稳定地黏附到水凝胶上。
