Fadl Hanaa O, Amin Noha M, Wanas Hanaa, El-Din Shimaa Saad, Ibrahim Heba A, Aboulhoda Basma Emad, Bocktor Nardeen Zakka
Medical Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Pharmacology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
J Parasit Dis. 2020 Dec;44(4):737-747. doi: 10.1007/s12639-020-01245-1. Epub 2020 Jul 25.
The role of nitric oxide (NO) in the immunopathological response during () infection remains controversial. The amino acid, l-arginine is a NO precursor commonly used by athletes and bodybuilders as a protein supplement. As to our knowledge, there are no published studies which have tested the effect of l-arginine on the intestinal phase of experimental trichinellosis. The present work aims to investigate the effect of l-arginine on the enteral phase of experimental infection in albendazole-treated and untreated mice. Forty BALB/C mice infected orally with larvae were divided into 4 groups as follows: Group A were infected and untreated (control) mice, Group B received albendazole alone, Group C received l-arginine alone, and Group D received both l-arginine and albendazole. Compared to the control group, l-arginine supplementation showed; a significant increase in the intestinal adult worm burden, a significantly high inducible NO synthase (iNOS) expression, elevated immune markers; tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), and enhanced apoptosis. Albendazole treated-group had a significant reduction in the adult worm number (90.9%), while combined albendazole-arginine regimen showed a lower percentage of worm reduction (72.7%). During the enteral phase of infection, l-arginine supplementation should be taken cautiously, as it may modulate the proinflammatory immune response and subsequently affect the outcome of the infection and/or treatment.
一氧化氮（NO）在（）感染期间免疫病理反应中的作用仍存在争议。氨基酸L-精氨酸是一种一氧化氮前体，运动员和健美运动员常用其作为蛋白质补充剂。据我们所知，尚无已发表的研究测试过L-精氨酸对实验性旋毛虫病肠道期的影响。本研究旨在调查L-精氨酸对阿苯达唑治疗和未治疗小鼠实验性（）感染肠内期的影响。将40只经口感染（）幼虫的BALB/C小鼠分为以下4组：A组为感染且未治疗的小鼠（对照组），B组仅接受阿苯达唑，C组仅接受L-精氨酸，D组同时接受L-精氨酸和阿苯达唑。与对照组相比，补充L-精氨酸显示：肠道成虫负荷显著增加，诱导型一氧化氮合酶（iNOS）表达显著升高，免疫标志物肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）升高，且细胞凋亡增强。阿苯达唑治疗组的成虫数量显著减少（90.9%），而阿苯达唑-精氨酸联合用药方案的驱虫率较低（72.7%）。在（）感染的肠内期，应谨慎补充L-精氨酸，因为它可能调节促炎免疫反应，进而影响感染和/或治疗的结果。
