College of Engineering, China Pharmaceutical University, No. 24 Tongjia Alley, Nanjing, P. R. China.
School of Science, China Pharmaceutical University, No. 24 Tongjia Alley, Nanjing, P. R. China.
J Mater Chem B. 2020 Dec 23;8(48):10966-10976. doi: 10.1039/d0tb02146f.
In this paper, a new magnetic molecular imprinted polymer-cyclodextrin (MMIP-CD) material was prepared by connecting β-cyclodextrin (CD) on the surface of a magnetic molecular imprinted polymer (MMIP) and used for the rapid and specific adsorption of zearalenone (ZEN). By using warfarin as the virtual template molecule, tetraethyl orthosilicate (TEOS) as the crosslinking agent, and (3-aminopropyl) triethoxysilane (APTES) as the functional monomer, a MMIP was produced by surface imprinting technology. Sulfobutyl ether-β-cyclodextrin attached to the surface of the MMIP under heating conditions produced a new specific adsorption material with exceptional adsorption capacity and excellent selectivity for ZEN. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and TEM-mapping results showed that the prepared MMIP-CD had a uniform particle size of about 480 nm, and the molecularly imprinted layer was successfully wrapped on the surface of the nanoparticles with a thickness of about 50 nm, whereby the cyclodextrin was effectively attached to the surface of the MMIP. The adsorption mechanism of MMIP-CD was confirmed by kinetic adsorption and thermodynamic adsorption experiments, the maximum adsorption capacity was found to be about 30 mg g-1, and the adsorption equilibrium could be reached within 20 min. The value of IF (QMMIP-CD/QMNIP) is 4.642. This showed that compared with MNIP, MMIP-CD showed a greatly improved specific adsorption capacity of ZEN. Selective experiments proved that MMIP-CD effectively combined the advantages of MMIP and CD, enhancing the adsorption capacity together with reducing the disadvantages that MMIP cannot distinguish structural analogs and CD cannot identify hydrophobic compounds effectively. In actual sample testing, the limit of quantification (LOQ) and limit of detection (LOD) were 0.1 ng kg-1 and 0.3 ng kg-1, respectively. The stability and detection precision of this method were 0.98-2.76% and 1.67-3.88%, respectively. The results proved that MMIP-CD had good development potential in the field of selective adsorption of ZEN, and laid the foundation for follow-up research.
本文通过在磁性分子印迹聚合物（MMIP）表面连接β-环糊精（CD），制备了一种新型的磁性分子印迹聚合物-环糊精（MMIP-CD）材料，用于快速、特异性吸附玉米赤霉烯酮（ZEN）。以华法林为虚拟模板分子，正硅酸乙酯（TEOS）为交联剂，（3-氨丙基）三乙氧基硅烷（APTES）为功能单体，采用表面印迹技术制备 MMIP。在加热条件下，将磺丁基醚-β-环糊精连接到 MMIP 表面，制得一种新的特异性吸附材料，对 ZEN 具有优异的吸附容量和选择性。扫描电子显微镜（SEM）、透射电子显微镜（TEM）和 TEM 图谱结果表明，所制备的 MMIP-CD 具有约 480nm 的均匀粒径，印迹层成功地包裹在约 50nm 厚的纳米颗粒表面，从而有效地将环糊精连接到 MMIP 表面。通过动力学吸附和热力学吸附实验证实了 MMIP-CD 的吸附机制，发现最大吸附容量约为 30mg/g，20min 内即可达到吸附平衡。IF（QMMIP-CD/QMNIP）值为 4.642，表明与 MNIP 相比，MMIP-CD 对 ZEN 的特异性吸附能力有了显著提高。选择性实验证明，MMIP-CD 有效结合了 MMIP 和 CD 的优势，提高了吸附容量，同时减少了 MMIP 无法区分结构类似物和 CD 无法有效识别疏水性化合物的缺点。在实际样品测试中，定量限（LOQ）和检测限（LOD）分别为 0.1ng/kg 和 0.3ng/kg。该方法的稳定性和检测精密度分别为 0.98-2.76%和 1.67-3.88%。结果表明，MMIP-CD 在 ZEN 选择性吸附领域具有良好的发展潜力，为后续研究奠定了基础。
