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对正常、受刺激及免疫激活的小鼠巨噬细胞的体外研究。I. 单层培养的巨噬细胞对1-¹⁴C葡萄糖的氧化作用。

In vitro studies on normal, stimulated and immunologically activated mouse macrophages. I. Oxidation of 1-14C glucose by macrophages in monolayer cultures.

作者信息

Riisgaard S, Bennedsen J, Rhodes J M

出版信息

Acta Pathol Microbiol Scand C. 1977 Aug;85C(4):233-8. doi: 10.1111/j.1699-0463.1977.tb03636.x.

DOI:10.1111/j.1699-0463.1977.tb03636.x
PMID:331865
Abstract

Oxidation of 1-14C glucose of BCG/listeria activated, proteose-peptone stimulated and typhoid-parathypoid vaccine (T.A.B.) stimulated peritoneal and spleen macrophages from C3H, CBA and C57Bl mice was determined at various times after treatment. The study showed no major differences between the oxidation of stimulated and activated macrophages, but a variation was seen in C3H mice in the kinetics of glucose oxidation of BCG/listeria activated and proteose-peptone stimulated peritoneal macrophages. It was also observed, in these mice, that the response appeared and disappeared earlier in the spleen than in the peritoneal macrophages.

摘要

在处理后的不同时间,测定了卡介苗/李斯特菌激活、蛋白胨刺激以及伤寒-副伤寒疫苗(T.A.B.)刺激的来自C3H、CBA和C57Bl小鼠的腹腔和脾脏巨噬细胞对1-¹⁴C葡萄糖的氧化情况。研究表明,刺激的巨噬细胞和激活的巨噬细胞在氧化方面没有重大差异,但在C3H小鼠中,卡介苗/李斯特菌激活的和蛋白胨刺激的腹腔巨噬细胞的葡萄糖氧化动力学存在差异。在这些小鼠中还观察到,脾脏中的反应比腹腔巨噬细胞中的反应出现得早且消失得早。

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引用本文的文献

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Correlation between in vivo and in vitro functional tests for activated macrophages.活化巨噬细胞的体内和体外功能测试之间的相关性
Infect Immun. 1979 Jan;23(1):34-40. doi: 10.1128/iai.23.1.34-40.1979.