两种露尾甲害虫线粒体全基因组的特征分析及其系统发育意义

Characterization of the Complete Mitochondrial Genomes from Two Nitidulid Pests with Phylogenetic Implications.

作者信息

Chen Xiaoxiao, Song Qing, Huang Min

机构信息

Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.

出版信息

Insects. 2020 Nov 11;11(11):779. doi: 10.3390/insects11110779.

DOI:10.3390/insects11110779

PMID:33187054

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7697951/

Abstract

The complete mitochondrial genomes of and sp. were sequenced and analyzed. The total genome lengths are 17,657 and 16,641 bp, with an A+T content of 77.2% and 76.4%, respectively. Each mitochondrial genome consists of 37 coding genes and a non-coding (AT-rich) region. All protein-coding genes (PCGs) start with the standard start codon, ATN, and end with complete stop codons, TAA and TAG, or an incomplete stop codon, T. All tRNAs can be folded into the typical clover-leaf secondary structure, with the exception of in both species with a reduced dihydrouridine (DHU) arm. The AT-rich region has tandem repeats differing in both number and length. Genetic distance and Ka/Ks analyses show that has a higher variability and more rapid evolutionary rate than other PCGs. Both maximum likelihood and Bayesian inference phylogenetic analyses based on 13 PCGs and 2 ribosome DNAs (rDNAs) agree with the previous phylogenies in supporting the Nitidulidae monophyly and the sister-group relationship of Kateretidae + (Monotomidae + Nitidulidae).

摘要

对[物种名称1]和[物种名称2] sp.的完整线粒体基因组进行了测序和分析。基因组总长度分别为17,657和16,641 bp，A+T含量分别为77.2%和76.4%。每个线粒体基因组由37个编码基因和一个非编码（富含AT）区域组成。所有蛋白质编码基因（PCGs）均以标准起始密码子ATN起始，并以完整终止密码子TAA和TAG或不完整终止密码子T结束。所有tRNA均可折叠成典型的三叶草二级结构，但这两个物种中的[某种tRNA名称]除外，其双氢尿嘧啶（DHU）臂有所缩短。富含AT的区域具有数量和长度均不同的串联重复序列。遗传距离和Ka/Ks分析表明，[基因名称]比其他PCGs具有更高的变异性和更快的进化速率。基于13个PCGs和2个核糖体DNA（rDNAs）的最大似然法和贝叶斯推断系统发育分析均与先前的系统发育结果一致，支持露尾甲科的单系性以及扁甲科+（隐食甲科+露尾甲科）的姐妹群关系。