Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, China.
Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China.
Int J Biol Macromol. 2019 Dec 1;141:29-40. doi: 10.1016/j.ijbiomac.2019.08.222. Epub 2019 Aug 27.
The complete mitochondrial genomes (mitogenomes) of Aphaena (Callidepsa) amabilis and Aphaena (Aphaena) discolor nigrotibiata were sequenced. The mitogenomes of these two species are 16,237 bp and 16,116 bp in length with an A + T content of 77.9% and 77.0%, respectively. Each contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a control region (A + T-rich region). All PCGs initiate with the standard start codon of ATN and terminate with the complete stop codon of TAA or TAG except for atp6, where nad1 ends with an incomplete T codon. All tRNAs have the typical clover-leaf structure except for trnS1 and trnV which have a reduced DHU arm. Moreover, these two mitogenomes have trnL2, trnR and trnT with an unpaired base in the acceptor stem. The putative A + T-rich region includes multiple types of tandem repeat regions. These phylogenetic analyses are reconstructed based on 13 protein-coding genes of 25 auchenorrhynchan mitogenomes, with both maximum likelihood and Bayesian analyses yielding robust identical phylogenetic trees. These results support a monophyletic Auchenorrhyncha and the relationship (Pyrops + (Lycorma + Aphaena)) within Fulgoridae.
已对 Aphaena (Callidepsa) amabilis 和 Aphaena (Aphaena) discolor nigrotibiata 的完整线粒体基因组（mitogenomes）进行了测序。这两个物种的线粒体基因组长度分别为 16,237bp 和 16,116bp，A+T 含量分别为 77.9%和 77.0%。每个基因组都包含 13 个蛋白编码基因（PCGs）、22 个转移 RNA 基因（tRNAs）、两个核糖体 RNA 基因（rRNAs）和一个控制区（富含 A+T 的区域）。所有 PCGs 均以 ATN 作为标准起始密码子开始，以 TAA 或 TAG 作为完整终止密码子结束，除了 atp6，nad1 以不完整的 T 密码子结束。所有 tRNAs 都具有典型的三叶形结构，除了 trnS1 和 trnV，它们的 DHU 臂较短。此外，这两个线粒体基因组中的 trnL2、trnR 和 trnT 在接受茎上有一个未配对的碱基。假定的富含 A+T 的区域包含多种类型的串联重复区域。这些系统发育分析是基于 25 种半翅目线粒体基因组的 13 个蛋白编码基因重建的，最大似然法和贝叶斯分析都产生了稳健的相同系统发育树。这些结果支持了半翅目是单系的，以及 Pyrrhocoridae 内的 Pyrops+（Lycorma+Aphaena）关系。
