Mao Xinyi, Wu Yuxiang, Chen Huitian, Wang Yifan, Yu Binger, Shi Guoqing
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, P. R.China.
Shandong Lvdu Biotechnology Co., Ltd, Shandong 256600, P. R.China.
Anal Methods. 2020 Dec 7;12(46):5628-5634. doi: 10.1039/d0ay01752c.
The common methods to detect zearalenone (ZEN) in edible oils need organic solvents to extract ZEN and then some sample purification process before detection, so, it is not convenient for on-site use. Here a simple method without organic solvents and a sample purification process was developed for the determination of ZEN in edible oils. The detection process only needs mixing oil with a surfactant solution in the indicated ratio and then loading the mixture onto a colloidal gold immunochromatographic (CGI) strip for detection. The optimized surfactant was AEO15 among the seven surfactants studied in this paper. The ZEN residue in edible oil could be quantitatively determined with a detection limit of 44.3 ng g-1, and the working range of the standard curve was from 50 to 800 ng g-1. This method has been successfully applied to the detection of ZEN in plant oils with recoveries ranging from 81 ± 7% to 129 ± 9% for spiked samples. The detection results for the ZEN residue in oil samples from a local market by this method were in good agreement with those obtained by the national standard method.
食用油中玉米赤霉烯酮（ZEN）的常用检测方法需要使用有机溶剂提取ZEN，然后在检测前进行一些样品净化处理，因此，不方便现场使用。本文开发了一种无需有机溶剂和样品净化过程的简单方法来测定食用油中的ZEN。检测过程只需将油与表面活性剂溶液按指定比例混合，然后将混合物加载到胶体金免疫层析（CGI）条上进行检测。在本文研究的七种表面活性剂中，优化后的表面活性剂为AEO15。食用油中的ZEN残留量可定量测定，检测限为44.3 ng g-1，标准曲线的工作范围为50至800 ng g-1。该方法已成功应用于植物油中ZEN的检测，加标样品的回收率为81±7%至129±9%。用该方法对当地市场油样中ZEN残留量的检测结果与国家标准方法的检测结果吻合良好。
