血清和外周动脉疾病患者动脉周围脂肪组织中 Sfrp5/Wnt5a 和瘦素/脂联素水平。
Sfrp5/Wnt5a and leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease.
机构信息
The Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
The Department of Medical Administration, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
出版信息
Clin Biochem. 2021 Jan;87:46-51. doi: 10.1016/j.clinbiochem.2020.11.002. Epub 2020 Nov 12.
BACKGROUND
Leptin, adiponectin, secreted frizzled-related protein 5 (Sfrp5) and wingless-type family member 5a (Wnt5a) are novel adipokines that are involved in insulin sensitivity and atherosclerosis. The aim of the present study was to investigate the serum and periarterial adipose tissue leptin/adiponectin and Sfrp5/Wnt5a levels in patients with peripheral arterial occlusive disease (PAOD).
METHODS
A total of 75 patients with PAOD and 39 control subjects were recruited. The serum concentrations of leptin, adiponectin, Sfrp5 and Wnt5a were measured by ELISAs, and the leptin, adiponectin, Sfrp5 and Wnt5a levels in the periarterial adipose tissue were observed by western blotting.
RESULTS
The serum Sfrp5 levels were significantly lower in the patients with PAOD than in the control subjects (p < 0.001) and Wnt5a levels were higher in the patients with PAOD (p < 0.001). The serum leptin levels were significantly higher in the patients with PAOD than in the control subjects (p < 0.001), and adiponectin levels were significantly lower in the patients with PAOD (p < 0.001). The serum Sfrp5 levels were associated with ABI (rs = 0.274; p = 0.018), Wnt5a (rs = -0.409; p < 0.001), adiponectin (rs = 0.244; p = 0.035) and Leptin/Adiponetin ratio (rs = -0.244; p = 0.037). The adiponectin and Sfrp5 protein levels were decreased in the periarterial adipose tissue of patients with PAOD compared with control subjects. The leptin and Wnt5a protein levels were increased in the periarterial adipose tissue of patients with PAOD compared with control subjects.
CONCLUSION
We demonstrated that the adiponectin and Sfrp5 levels in the serum and periarterial adipose tissue were significantly lower in the patients with PAOD than in the control subjects. The leptin and Wnt5a levels in the serum and periarterial adipose tissue were significantly higher in the patients with PAOD than in the control subjects.
背景
瘦素、脂联素、分泌卷曲相关蛋白 5（Sfrp5）和无翅型 MMTV 整合位点家族成员 5a（Wnt5a）是新型脂肪因子，参与胰岛素敏感性和动脉粥样硬化。本研究旨在探讨外周动脉闭塞性疾病（PAOD）患者血清和动脉周围脂肪组织中瘦素/脂联素和 Sfrp5/Wnt5a 水平。
方法
共纳入 75 例 PAOD 患者和 39 例对照组。采用 ELISA 法检测血清瘦素、脂联素、Sfrp5 和 Wnt5a 浓度，采用 Western blot 法观察动脉周围脂肪组织中瘦素、脂联素、Sfrp5 和 Wnt5a 水平。
结果
PAOD 患者血清 Sfrp5 水平明显低于对照组（p<0.001），Wnt5a 水平明显高于对照组（p<0.001）。PAOD 患者血清瘦素水平明显高于对照组（p<0.001），脂联素水平明显低于对照组（p<0.001）。血清 Sfrp5 水平与 ABI（rs=0.274；p=0.018）、Wnt5a（rs=-0.409；p<0.001）、脂联素（rs=0.244；p=0.035）和瘦素/脂联素比值（rs=-0.244；p=0.037）相关。与对照组相比，PAOD 患者动脉周围脂肪组织中脂联素和 Sfrp5 蛋白水平降低，瘦素和 Wnt5a 蛋白水平升高。
结论
我们证实，PAOD 患者血清和动脉周围脂肪组织中脂联素和 Sfrp5 水平明显低于对照组，血清和动脉周围脂肪组织中瘦素和 Wnt5a 水平明显高于对照组。
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