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蒲公英(Taraxacum mongolicum)叶中的多糖:创新干燥技术对其结构特性和生物活性的影响。

Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.

机构信息

Institute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya'an 625014, Sichuan, China.

Institute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya'an 625014, Sichuan, China; Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, Sichuan, China.

出版信息

Int J Biol Macromol. 2021 Jan 15;167:995-1005. doi: 10.1016/j.ijbiomac.2020.11.054. Epub 2020 Nov 11.

Abstract

The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs). Five different DLPs were obtained from dandelion leaves dried by abovementioned drying techniques. Results showed that the structural characteristics and bioactivities of DLPs varied with different drying techniques. The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar. In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase. Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.

摘要

本研究旨在深入了解创新干燥技术(射频干燥和微波干燥)和传统干燥技术(真空干燥、冷冻干燥和热风干燥)对蒲公英叶多糖(DLPs)结构特征和生物活性的影响。通过上述干燥技术干燥蒲公英叶后得到了五种不同的 DLPs。结果表明,DLPs 的结构特征和生物活性随干燥技术的不同而变化。不同干燥技术得到的 DLPs 的分子量、表观粘度、单糖组成摩尔比、糖醛酸含量和结合多酚含量均有明显差异,而 DLPs 中的单糖组成类型和主要糖苷键则相似。此外,研究结果表明,DLPs,特别是通过射频干燥得到的 DLP-RF,具有显著的抗氧化活性(ABTS、DPPH 和 NO 自由基清除活性)、优异的体外抗糖化活性和明显的体外α-葡萄糖苷酶抑制活性。本研究结果表明,射频干燥可作为提取 DLPs 前的一种潜在干燥技术,应用于功能性食品和医药行业。

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