Dandelion () Extract Alleviated HO-Induced Oxidative Damage: The Underlying Mechanism Revealed by Metabolomics and Lipidomics.

Dandelion has received wide attention in food and medicine fields due to its excellent antioxidant properties. Nonetheless, the underlying mechanism of this action has not yet been fully clarified, particularly at the metabolic level. Herein, the effects of dandelion extract (DE) on HO-induced oxidative damage was investigated. The results indicate that the DE alleviated HO-induced cell damage (increased by 14.5% compared to HO group), reduced the reactive oxygen species (ROS) level (decreased by 80.1% compared to HO group), maintained the mitochondrial membrane potential (MMP) level, and increased antioxidant-related enzyme activities. Importantly, the metabolic response of PC12 cells indicates that HO disturbed phospholipid metabolism and damaged cell membrane integrity. In addition, energy metabolism, the central nervous system, and the antioxidant-related metabolism pathway were perturbed. In contrast, DE rescued the HO-induced metabolic disorder and further alleviated oxidative damage. Collectively, these findings provide valuable stepping stones for a discussion of the mechanism and show the promise of DE as a suitable additive for functional food products.