He Menglu, Sun Hao, Wei Jianhua, Zhang Ran, Han Xiang'en, Ni Zhonghai
School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, People's Republic of China.
School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119138. doi: 10.1016/j.saa.2020.119138. Epub 2020 Nov 4.
It is very important to exploit real-time, ultrasensitive and specific visualization detection methods for hypochlorous acid/hypochlorite (HOCl/ClO) in biological systems as they are the guardians of the human immune system against pathogens invasion. In our work, we designed a novel reversible naphthalimide-based fluorescent probe NAP-OH to recognize HClO/ClO with a unique selective colorimetric and fluorescent response, a short response time (<8 s) and a high sensitivity (10.3 nM). In addition, NAP-OH exhibits a novel on-off-on fluorescence response to ClO/ascorbic acid (AA) with good cycle stability. The fluorescence signal is quenched because HClO/ClO oxidizes the subunit of NAP-OH to the segment 2,2,6,6-tetramethyl-1-oxo-piperidinium in NAP-O, which can be reduced by AA with the recovery of fluorescence. Finally, the confocal fluorescence imaging has been performed, which proves that NAP-OH can satisfactorily monitor intracellular endogenous and exogenous HClO/AA redox cycles.
开发用于生物系统中次氯酸/次氯酸盐（HOCl/ClO）的实时、超灵敏和特异性可视化检测方法非常重要，因为它们是人类免疫系统抵御病原体入侵的守护者。在我们的工作中，我们设计了一种基于萘酰亚胺的新型可逆荧光探针NAP-OH，用于识别HClO/ClO，具有独特的选择性比色和荧光响应、短响应时间（<8秒）和高灵敏度（10.3 nM）。此外，NAP-OH对ClO/抗坏血酸（AA）表现出新颖的开-关-开荧光响应，具有良好的循环稳定性。荧光信号被淬灭是因为HClO/ClO将NAP-OH的亚基氧化为NAP-O中的2,2,6,6-四甲基-1-氧代哌啶鎓片段，该片段可被AA还原并恢复荧光。最后，进行了共聚焦荧光成像，证明NAP-OH可以令人满意地监测细胞内源性和外源性HClO/AA的氧化还原循环。
