Ding Feng, Wen Han, Zhuo Ruijiang, Li Jiawei, Zheng Hui, Deng Yuru, Shen Jianliang, He Xiaojun
Department of Microbiology & Immunology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Anal Chim Acta. 2021 May 29;1161:338472. doi: 10.1016/j.aca.2021.338472. Epub 2021 Apr 5.
Hypochlorous acid (HClO) along with its ionic form, hypochlorite anion (ClO) are critical reactive oxygen species (ROS), which play vital roles in biological systems. Dysregulated production of HClO/ClO can result in tissue damage and cause a variety of diseases. Besides, Sodium hypochlorite has been widely used as a bleaching agent for water disinfection, surface cleaning in daily life. Excessive exposure to sodium hypochlorite will lead to symptoms of severe breathing and skin problems. Therefore, developing a state-of-the-art (simple, highly sensitive, highly selective and super fast-response) sensor for tracking HClO is of biological, toxicological, and environmental importance. Though many HClO probes have been reported so far, this big aim still presents a challenge. Researchers around the world are continuing to develop new HClO probes that could improve their sensitivity, selectivity, the limit of detection, response time, easiness to use, etc. Herein, with coumarin as the fluorophore molecule, we rationally developed a novel chemosensor (CMTH) for detecting HClO with both ratiometric and colorimetric responses resulted from the oxidation reaction of CN bond. Further analysis results indicated that CMTH can realize highly sensitive with low limit of detection (256 nM, among the best of its kind) and highly selective (over a bunch of interfering analytes) imaging detection of HClO in multiple organisms with low cytotoxicity, and good cell and tissue permeability as well. In particular, compared to other fluorescent HClO probes reported so far, CMTH excels in the response time to HClO (< 40 s), being the top-notch of its kind. Besides, owing to its excellent water solubility, CMTH can also be applied to track HClO in the environmental system. Taken together, we have presented here a novel chemosensor, CMTH, as a colorimetric and ratiometric chemosensor for highly sensitive and ultrafast imaging detection of HClO in aqueous solutions, eukaryotic cells, prokaryotic bacteria and vertebrate zebrafish.
次氯酸（HClO）及其离子形式次氯酸根阴离子（ClO）是关键的活性氧物种（ROS），在生物系统中发挥着至关重要的作用。HClO/ClO生成失调会导致组织损伤并引发多种疾病。此外，次氯酸钠已被广泛用作水消毒的漂白剂、日常生活中的表面清洁剂。过度接触次氯酸钠会导致严重的呼吸症状和皮肤问题。因此，开发一种先进的（简单、高灵敏度、高选择性和超快速响应）用于追踪HClO的传感器具有生物学、毒理学和环境重要性。尽管到目前为止已经报道了许多HClO探针，但实现这一宏大目标仍然是一项挑战。世界各地的研究人员正在继续开发新的HClO探针，以提高其灵敏度、选择性、检测限、响应时间、易用性等。在此，以香豆素作为荧光团分子，我们合理地开发了一种新型化学传感器（CMTH），用于检测HClO，其基于CN键的氧化反应产生比率和比色响应。进一步的分析结果表明，CMTH能够以低检测限（256 nM，同类最佳之一）实现高灵敏度，并且对多种生物体中的HClO具有高选择性（对一系列干扰分析物）成像检测，具有低细胞毒性以及良好的细胞和组织渗透性。特别是，与迄今为止报道的其他荧光HClO探针相比，CMTH对HClO的响应时间极快（<40秒），在同类中名列前茅。此外，由于其出色的水溶性，CMTH还可应用于环境系统中追踪HClO。综上所述，我们在此展示了一种新型化学传感器CMTH，作为一种比色和比率化学传感器，用于在水溶液、真核细胞、原核细菌和脊椎动物斑马鱼中对HClO进行高灵敏度和超快速成像检测。
