Antiochia Riccarda
Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
Biosens Bioelectron. 2021 Feb 1;173:112777. doi: 10.1016/j.bios.2020.112777. Epub 2020 Oct 28.
This review summarizes the state of art of biosensor technology for Coronavirus (CoV) detection, the current challenges and the future perspectives. Three categories of affinity-based biosensors (ABBs) have been developed, depending on their transduction mechanism, namely electrochemical, optical and piezoelectric biosensors. The biorecognition elements include antibodies and DNA, which undergo important non-covalent binding interactions, with the formation of antigen-antibody and ssDNA/oligonucleotide-complementary strand complexes in immuno- and DNA-sensors, respectively. The analytical performances, the advantages and drawbacks of each type of biosensor are highlighted, discussed, and compared to traditional methods. It is hoped that this review will encourage scientists and academics to design and develop new biosensing platforms for point-of-care (POC) diagnostics to manage the coronavirus disease 2019 (COVID-19) pandemic, providing interesting reference for future studies.
本综述总结了用于冠状病毒（CoV）检测的生物传感器技术的现状、当前挑战及未来展望。根据其传感机制，已开发出三类基于亲和力的生物传感器（ABB），即电化学生物传感器、光学生物传感器和压电化学生物传感器。生物识别元件包括抗体和DNA，它们分别在免疫传感器和DNA传感器中通过重要的非共价结合相互作用，形成抗原-抗体复合物和单链DNA/寡核苷酸-互补链复合物。文中重点介绍、讨论了各类生物传感器的分析性能、优缺点，并与传统方法进行了比较。希望本综述能鼓励科学家和学者设计和开发用于即时检测（POC）诊断的新型生物传感平台，以应对2019冠状病毒病（COVID-19）大流行，为未来研究提供有益参考。
