Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Iranshahr University Medical Sciences, Iranshahr, Iran.
Comp Immunol Microbiol Infect Dis. 2021 Feb;74:101573. doi: 10.1016/j.cimid.2020.101573. Epub 2020 Nov 3.
Hydatidosis is one of the most important zoonotic diseases with a worldwide distribution and it seems that the survival of Echinococcus granulosus in nature for many years, is due to having different mechanisms to escape from the host immune systems. One of these efficient mechanisms is the production of various antigens and proteins by the larva of the parasite and the main purpose of this study is evaluation of manifestation of various antigens in different parts of intermediate host. The hepatic and pulmonary hydatid cysts were gathered from sheep and the antigens of different parts of the cysts (laminated layer, protoscolices and cyst fluid) were separated and analyzed by SDS-PAGE and then transferred to nitrocellulose paper and finally, Western blot analysis was evaluated the immunogenicity of proteins. The antigens of laminated layer, protoscolices and hydatid cyst fluid, in different tissues of the liver and lungs, manifest various proteins and also these antigens are immunogenically different. Also, it is found more immunogenic proteins in the laminated layer than the other parts of the cysts. The various proteins are generated by Echinococcus granulosus larva depending on the type of tissues attacked by the parasite. Increasing the chance of survival may be the main cause of manifestation various antigens in different parts of cysts and host tissues. These antigenic variations might have made diagnostic serologic test unreliable.
包虫病是一种具有全球分布的最重要的人畜共患疾病之一，似乎细粒棘球绦虫在自然界中多年来的生存，是由于具有不同的机制来逃避宿主的免疫系统。其中一个有效的机制是由幼虫寄生虫产生各种抗原和蛋白质，本研究的主要目的是评估各种抗原在中间宿主不同部位的表现。从绵羊中收集肝和肺包虫囊肿，分离和分析囊的不同部位（生发层、原头蚴和囊液）的抗原，通过 SDS-PAGE 转移到硝酸纤维素纸，最后，通过 Western blot 分析评估蛋白质的免疫原性。在肝和肺的不同组织中，生发层、原头蚴和包虫囊液的抗原表现出不同的蛋白质，而且这些抗原在免疫原性上也不同。此外，在生发层中发现了比其他囊部分更多的免疫原性蛋白。不同的蛋白质是由细粒棘球绦虫幼虫产生的，这取决于寄生虫攻击的组织类型。增加生存机会可能是在囊和宿主组织的不同部位表现出各种抗原的主要原因。这些抗原的变化可能使诊断血清学试验不可靠。
