Synthetic rEg.P29 Peptides Induce Protective Immune Responses Against in Mice.

represents a significant threat to animal husbandry and human health, but its consequences are often underestimated. Vaccination can prevent infection. We investigated the immune protective effect induced by the recombinant protein P29 of (rEg.P29) peptide vaccine. : The CD4 T-, CD8 T-, Treg-, and CD8CD107a T-cell proportions in the spleen and peripheral blood of infected mice were analyzed using flow cytometry. Additionally, we measured the proportions of IFN-γ and IL-2 secreted by memory T cells, CD19CD138B cells, CD19CD138 plasmablasts, CD19CD138 plasma cells, and CD19IgDIgG and CD19IgDIgA memory B cells. : No significant differences were noted in CD4 T-, CD8 T-, and CD8CD107a Treg-cell percentages among the experimental groups. However, IFN-γ, IL-2, and TNF-α levels and vaccine-specific antibody concentrations in the plasma were significantly elevated in the rEg.P29 + CpG + infection and rEg.P29 + CpG + infection groups compared to those in the PBS + infection and CpG + infection groups. Similarly, CD19CD138 plasma cell and CD19IgDIgG and CD19IgDIgA memory B-cell populations, along with specific antibodies, were significantly higher in these groups. Especially, the average cyst burden in the rEg.P29 + CpG + infection and rEg.P29 + CpG + infection groups was significantly reduced compared to that in the PBS + infection and CpG + infection groups. : Synthetic peptide vaccines targeting rEg.P29 can effectively inhibit cysts, offering a novel strategy for the development of vaccines against . These findings provide a foundation for further research on the immunogenicity and protective efficacy of rEg.P29-based vaccines.