Henan Provincial Ecological Planting Engineering Technology Research Center of Daodi Herbs, School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Co-Construction Collaborative Innovation Centre for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of PR China, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Molecules. 2022 Aug 25;27(17):5470. doi: 10.3390/molecules27175470.
is an important medicinal plant in China; however, little is known regarding how this plant transcribes and synthesizes saikosaponins under drought stress. Herein, we investigated how drought stress stimulates the transcriptional changes of to synthesize saikosaponins. Short-term drought stress induced the accumulation of saikosaponins, especially from the first re-watering stage (RD_1 stage) to the second re-watering stage (RD_2 stage). Saikosaponin-a and saikosaponin-d increased by 84.60% and 75.13%, respectively, from the RD_1 stage to the RD_2 stage. Drought stress also stimulated a rapid increase in the levels of the hormones abscisic acid, salicylic acid, and jasmonic acid. We screened 49 Unigenes regarding the terpenoid backbone and triterpenoid biosynthesis, of which 33 differential genes were significantly up-regulated during drought stress. Moreover, one P450 and two UGTs are possibly involved in the synthesis of saikosaponins, while some transcription factors may be involved in regulating the expression of key enzyme genes. Our study provides a reference for the cultivation of and a practical means to ensure the quality (safety and effectiveness) of for medicinal use, as well as insights into the modernization of the China Agriculture Research System.
是中国的一种重要药用植物；然而，关于该植物在干旱胁迫下如何转录和合成柴胡皂苷知之甚少。本文研究了干旱胁迫如何刺激转录变化来合成柴胡皂苷。短期干旱胁迫诱导柴胡皂苷的积累，特别是从第一次复水阶段（RD_1 阶段）到第二次复水阶段（RD_2 阶段）。柴胡皂苷-a 和柴胡皂苷-d 分别增加了 84.60%和 75.13%，从 RD_1 阶段到 RD_2 阶段。干旱胁迫还刺激了激素脱落酸、水杨酸和茉莉酸水平的快速增加。我们筛选了 49 个涉及萜类骨架和三萜类生物合成的 Unigenes，其中 33 个差异基因在干旱胁迫下显著上调。此外，一个 P450 和两个 UGTs 可能参与柴胡皂苷的合成，而一些转录因子可能参与调节关键酶基因的表达。本研究为的栽培提供了参考，也为确保药用的质量（安全性和有效性）提供了实用手段，并为中国农业研究系统的现代化提供了思路。
