Winter Rafael Contage, Antunes Apio Claudio Martins, de Oliveira Francine Hehn
Departments of Neurosurgery Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
Departments of Neurosurgery Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
Surg Neurol Int. 2020 Oct 8;11:328. doi: 10.25259/SNI_528_2020. eCollection 2020.
Meningioma is the most common benign intracranial neoplasm, accounting for 30% of all primary brain tumors. In 90% of cases, meningiomas are benign. Several aspects of molecular biology, including potential biomarkers, have been studied in attempts to better understand the natural history of meningiomas. Vascular endothelial growth factor (VEGF) is a biomarker responsible for inducing physiological and pathological angiogenesis. VEGF expression has been investigated as a potential predictor of several tumor aspects, including growth rate, recurrence rate, brain tissue invasion, peritumoral edema and surgical prognosis, and also as a marker of histological grade. However, there is no consensus in the literature with respect to the association between this biological factor and meningioma. We digitally analyzed immunohistochemical images using ImageJ software with the aim of correlating VEGF expression with tumor histology.
Tissue samples from patients presenting with meningioma who had undergone surgical removal between 2007 and 2016 at the Hospital de Clínicas de Porto Alegre (HCPA), in Southern Brazil, were analyzed to identify possible immunohistochemical associations between VEGF and histological grade and subtype.
Seventy-six patients were included; 82% were female, mean age was 59.9 years (range: 18-91). No statistically significant associations were found between VEGF expression and histological grade or subtype ( = 0.310).
Our findings suggest that VEGF is frequently present in meningiomas regardless of histological grade and should not be used as a marker of severity or histological grade.
脑膜瘤是最常见的颅内良性肿瘤，占所有原发性脑肿瘤的30%。在90%的病例中，脑膜瘤是良性的。为了更好地了解脑膜瘤的自然病史，人们对分子生物学的几个方面，包括潜在的生物标志物进行了研究。血管内皮生长因子（VEGF）是一种负责诱导生理性和病理性血管生成的生物标志物。VEGF表达已被作为包括生长速率、复发率、脑组织侵袭、瘤周水肿和手术预后等几个肿瘤方面的潜在预测指标进行研究，同时也作为组织学分级的标志物。然而，关于这种生物学因素与脑膜瘤之间的关联，文献中尚无共识。我们使用ImageJ软件对免疫组化图像进行数字分析，目的是将VEGF表达与肿瘤组织学相关联。
对2007年至2016年期间在巴西南部阿雷格里港临床医院（HCPA）接受手术切除的脑膜瘤患者的组织样本进行分析，以确定VEGF与组织学分级和亚型之间可能的免疫组化关联。
纳入76例患者；82%为女性，平均年龄59.9岁（范围：18 - 91岁）。未发现VEGF表达与组织学分级或亚型之间存在统计学显著关联（ = 0.310）。
我们的研究结果表明，无论组织学分级如何，VEGF在脑膜瘤中经常存在，不应将其用作严重程度或组织学分级的标志物。
