Lachowski Dariusz, Cortes Ernesto, Matellan Carlos, Rice Alistair, Lee David A, Thorpe Stephen D, Del Río Hernández Armando E
Cellular and Molecular Biomechanics Laboratory, Department of Bioengineering, Imperial College London, London, United Kingdom.
Institute of Bioengineering, School of Engineering and Material Science, Queen Mary University of London, London, United Kingdom.
Front Cell Dev Biol. 2020 Oct 22;8:592628. doi: 10.3389/fcell.2020.592628. eCollection 2020.
Mechanical forces regulate cell functions through multiple pathways. G protein-coupled estrogen receptor (GPER) is a seven-transmembrane receptor that is ubiquitously expressed across tissues and mediates the acute cellular response to estrogens. Here, we demonstrate an unidentified role of GPER as a cellular mechanoregulator. G protein-coupled estrogen receptor signaling controls the assembly of stress fibers, the dynamics of the associated focal adhesions, and cell polarization RhoA GTPase (RhoA). G protein-coupled estrogen receptor activation inhibits F-actin polymerization and subsequently triggers a negative feedback that transcriptionally suppresses the expression of monomeric G-actin. Given the broad expression of GPER and the range of cytoskeletal changes modulated by this receptor, our findings position GPER as a key player in mechanotransduction.
机械力通过多种途径调节细胞功能。G蛋白偶联雌激素受体（GPER）是一种七跨膜受体，在各组织中广泛表达，介导细胞对雌激素的急性反应。在此，我们证明了GPER作为一种细胞机械调节因子的未知作用。G蛋白偶联雌激素受体信号传导控制应力纤维的组装、相关粘着斑的动力学以及细胞极化的RhoA GTP酶（RhoA）。G蛋白偶联雌激素受体激活抑制F-肌动蛋白聚合，随后触发负反馈，转录抑制单体G-肌动蛋白的表达。鉴于GPER的广泛表达以及该受体调节的细胞骨架变化范围，我们的研究结果将GPER定位为机械转导中的关键参与者。
