State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, PR China.
Present address: Shanxi Eye Hospital, Taiyuan, PR China.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004567. Epub 2020 Nov 16.
Six aerobic, non-motile, non-haemolytic, Gram-stain-negative, oxidase-negative strains (185, 187, 323-1, 194, dk386 and dk771) were recovered from different faecal samples of on the Qinghai-Tibet Plateau. In the 16S rRNA gene sequences, one strain pair, 185/187, shared highest similarity to 114 (97.9 %), and the other two (323-1/194 and dk771/dk386) to CGMCC 1.12528 (98.6 and 97.0 %, respectively). Phylogenomic tree analysis showed that these six strains formed three separate clades in the genus . Digital DNA-DNA hybridization values of each pair of the isolates with all members of the genus were far below 70 %. The main cellular fatty acids of all six strains were C 9, C and summed feature 3 (C 7/C 6). Q-9 was the predominant respiratory quinone for strains 185, 323-1 and dk386. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the genotypic, phenotypic and biochemical analyses, these six strains represent three novel species of the genus , for which the names sp. nov., sp. nov. and sp. nov. are proposed. The type strains are 185 (=CGMCC 1.13636=JCM 33607), 323-1 (=CGMCC 1.13940=JCM 33608) and dk386 (=CGMCC 1.16589=JCM 33592), respectively.
从青藏高原不同的粪便样本中分离到 6 株好氧、非运动、非溶血、革兰氏阴性、氧化酶阴性的菌株（185、187、323-1、194、dk386 和 dk771）。在 16S rRNA 基因序列中，一对菌株 185/187 与 114 （97.9%）相似度最高，另外两株（323-1/194 和 dk771/dk386）与 CGMCC 1.12528（98.6%和 97.0%）相似度最高。系统发育树分析表明，这 6 株菌在 属中形成 3 个独立的分支。每对分离株与属内所有成员的数字 DNA-DNA 杂交值均远低于 70%。所有 6 株菌的主要细胞脂肪酸均为 C9、C 和总和特征 3（C7/C6）。菌株 185、323-1 和 dk386 的主要呼吸醌为 Q-9。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。基于基因型、表型和生化分析，这 6 株菌代表了 属的 3 个新种，分别命名为 sp. nov.、 sp. nov.和 sp. nov.。模式菌株分别为 185（=CGMCC 1.13636=JCM 33607）、323-1（=CGMCC 1.13940=JCM 33608）和 dk386（=CGMCC 1.16589=JCM 33592）。
