Bocckino S B, Wilson P B, Exton J H
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232.
FEBS Lett. 1987 Dec 10;225(1-2):201-4. doi: 10.1016/0014-5793(87)81157-2.
Vasopressin, angiotensin II and epinephrine elicited the accumulation of phosphatidylethanol in rat hepatocytes exposed to ethanol and of phosphatidate in the absence of ethanol. When isolated liver plasma membranes were exposed to ethanol, GTP gamma S stimulated the production of phosphatidylethanol whereas phosphatidate was formed in the absence of ethanol. With increasing ethanol concentrations, phosphatidate formation declined whereas phosphatidylethanol production increased. These findings suggest that rat hepatocytes possess a hormone-dependent phospholipase D activity that can also catalyze the formation of phosphatidylethanol.
血管加压素、血管紧张素II和肾上腺素可促使暴露于乙醇的大鼠肝细胞中磷脂酰乙醇的积累，以及在无乙醇情况下磷脂酸的积累。当分离的肝细胞膜暴露于乙醇时，GTPγS可刺激磷脂酰乙醇的产生，而磷脂酸则在无乙醇的情况下形成。随着乙醇浓度的增加，磷脂酸的形成减少，而磷脂酰乙醇的产生增加。这些发现表明，大鼠肝细胞具有一种激素依赖性磷脂酶D活性，该活性也可催化磷脂酰乙醇的形成。
