Department of Immunology, University of Washington, Seattle, Washington, USA.
UT Health San Antonio, Texas, USA.
Cytometry A. 2021 Jan;99(1):60-67. doi: 10.1002/cyto.a.24265. Epub 2020 Nov 24.
Data management is essential in a flow cytometry (FCM) shared resource laboratory (SRL) for the integrity of collected data and its long-term preservation, as described in the Cytometry publication from 2016, ISAC Flow Cytometry Shared Resource Laboratory (SRL) Best Practices (Barsky et al.: Cytometry Part A 89A(2016): 1017-1030). The SARS-CoV-2 pandemic introduced an array of challenges in the operation of SRLs. The subsequent laboratory shutdowns and access restrictions brought to the forefront well-established practices that withstood the impact of a sudden change in operations and illuminated areas that need improvement. The most significant challenges from a data management perspective were data access for remote analysis and workstation management. Notably, lessons learned from this challenge emphasize the importance of safeguarding collected data from loss in various emergencies such as fire or natural disasters where the physical hardware storing data could be directly affected. Here, we describe two data management systems that have been successful during the current emergency created by the pandemic, specifically remote access and automated data transfer. We will discuss other situations that could arise and lead to data loss or challenges in interpreting data. © 2020 International Society for Advancement of Cytometry.
数据管理对于流式细胞术(FCM)共享资源实验室(SRL)至关重要,以确保收集数据的完整性及其长期保存,正如 2016 年《细胞术》杂志上发表的 ISAC 流式细胞术共享资源实验室(SRL)最佳实践(Barsky 等人:细胞术部分 A 89A(2016):1017-1030)所述。SARS-CoV-2 大流行给 SRL 的运营带来了一系列挑战。随后的实验室关闭和访问限制凸显了一些久经考验的实践,这些实践经受住了运营突然变化的影响,并揭示了需要改进的领域。从数据管理的角度来看,最大的挑战是远程分析和工作站管理的数据访问。值得注意的是,从这一挑战中吸取的经验教训强调了保护收集的数据在各种紧急情况(如火灾或自然灾害)中免受丢失的重要性,在这些紧急情况中,存储数据的物理硬件可能会直接受到影响。在这里,我们描述了在当前由大流行引起的紧急情况下成功实施的两种数据管理系统,即远程访问和自动数据传输。我们将讨论可能出现的其他情况,这些情况可能导致数据丢失或数据解释方面的挑战。©2020 国际细胞术促进协会。