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The electroanalytical approach to lipid peroxide determinations.

作者信息

Funk M O

机构信息

Department of Chemistry, University of Toledo, OH 43606.

出版信息

Free Radic Biol Med. 1987;3(5):319-21. doi: 10.1016/s0891-5849(87)80039-4.

DOI:10.1016/s0891-5849(87)80039-4
PMID:3319796
Abstract

An electroanalytical method for the determination of lipid peroxides in physiological material is described. The technique is based on electrochemical detection for HPLC as the means for enhancing sensitivity. Samples containing organic peroxides, including lipid peroxides, can be analyzed directly using a modified polarographic detector (Lloyd, J.B.F.; Optimization of the operational parameters of the supported mercury drop electrode detector in high performance liquid chromatography. Anal. Chim. Acta 154:121-131; 1983.) for reversed phase HPLC determinations. Detection limits for fatty acid hydroperoxides were found to be in the low nanogram range.

摘要

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