Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry, Seoul 03722, Korea.
BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Korea.
Int J Mol Sci. 2020 Nov 12;21(22):8501. doi: 10.3390/ijms21228501.
Despite numerous advantages of using porous hydroxyapatite (HAp) scaffolds in bone regeneration, the material is limited in terms of osteoinduction. In this study, the porous scaffold made from nanosized HAp was coated with different concentrations of osteoinductive aqueous methylsulfonylmethane (MSM) solution (2.5, 5, 10, and 20%) and the corresponding MH scaffolds were referred to as MH2.5, MH5, MH10, and MH20, respectively. The results showed that all MH scaffolds resulted in burst release of MSM for up to 7 d. Cellular experiments were conducted using MC3T3-E1 preosteoblast cells, which showed no significant difference between the MH2.5 scaffold and the control with respect to the rate of cell proliferation ( > 0.05). There was no significant difference between each group at day 4 for alkaline phosphatase (ALP) activity, though the MH2.5 group showed higher level of activity than other groups at day 10. Calcium deposition, using alizarin red staining, showed that cell mineralization was significantly higher in the MH2.5 scaffold than that in the HAp scaffold ( < 0.0001). This study indicated that the MH2.5 scaffold has potential for both osteoinduction and osteoconduction in bone regeneration.
尽管多孔羟基磷灰石（HAp）支架在骨再生中有许多优势，但该材料在成骨诱导方面存在局限性。在这项研究中，纳米级 HAp 制成的多孔支架涂覆了不同浓度的成骨诱导性水甲基磺酰甲烷（MSM）溶液（2.5%、5%、10%和 20%），相应的 MH 支架分别命名为 MH2.5、MH5、MH10 和 MH20。结果表明，所有 MH 支架在长达 7 天内都导致 MSM 的爆发式释放。使用 MC3T3-E1 前成骨细胞进行细胞实验，MH2.5 支架与对照组的细胞增殖率没有显著差异（>0.05）。在第 4 天，碱性磷酸酶（ALP）活性方面，各组之间没有显著差异，尽管在第 10 天，MH2.5 组的活性水平高于其他组。茜素红染色显示，细胞矿化在 MH2.5 支架中明显高于 HAp 支架（<0.0001）。这项研究表明，MH2.5 支架在骨再生中有成骨诱导和骨传导的潜力。
