Lee Kyungwoo, Min Daejin, Choi Yonghyun, Yoon Semi, Jang Jaehee, Hwang Jangsun, Jeon Hojeong, Cho Yong Woo, Choi Jonghoon
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.
Center for Biomaterials, Korea Institute of Science and Technology, Seoul 02792, Korea.
Biomedicines. 2020 Nov 12;8(11):497. doi: 10.3390/biomedicines8110497.
We aimed to design and manufacture a transporter capable of delivering small interfering RNAs (siRNAs) into the skin without causing any damage. β-glucans are unique chiral polysaccharides with well-defined immunological properties and supramolecular wrapping ability. However, the chiral properties of these polymers have hardly been applied in drug delivery systems. In this study, β-glucan nanoparticles were designed and manufactured to deliver genetic material to the target cells. The β-glucan molecules were self-assembled with an siRNA into nanoparticles of 300-400 nm in diameter via a conformational transition process, in order to construct a gene delivery system. The assembled gene nanocarriers were associated with high gene-loading ability. The expression and efficiency of siRNA were verified after its delivery via β-glucan. Our results provide evidence that β-glucan nanoparticles can be effectively used to deliver siRNA into the cells.
我们旨在设计并制造一种能够将小干扰RNA（siRNA）递送至皮肤且不会造成任何损伤的转运体。β-葡聚糖是具有明确免疫特性和超分子包裹能力的独特手性多糖。然而，这些聚合物的手性特性在药物递送系统中几乎未得到应用。在本研究中，设计并制造了β-葡聚糖纳米颗粒以将遗传物质递送至靶细胞。β-葡聚糖分子通过构象转变过程与siRNA自组装成直径为300 - 400 nm的纳米颗粒，以构建基因递送系统。组装后的基因纳米载体具有高基因负载能力。在通过β-葡聚糖递送siRNA后，验证了其表达和效率。我们的结果提供了证据，表明β-葡聚糖纳米颗粒可有效地用于将siRNA递送至细胞中。
