Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.
Department of Pharmacology, Yonsei University College of Medicine, Seoul, South Korea.
Sci Rep. 2020 Nov 16;10(1):19915. doi: 10.1038/s41598-020-76467-7.
The roles of individual bacteria and their relationship in the development of colorectal cancer (CRC) remain unclear. We aimed to determine the prevalence of CRC-associated bacteria using quantitative real-time PCR (qPCR) or 16S rRNA analysis and the statistical correlations of patient demographics and clinical characteristics comprising alcohol consumption with CRC-associated bacteria. We determined the prevalence of five CRC-associated bacterial species in 38 CRC patients (39 samples) and 21 normal individuals using qPCR, and the relative abundance of bacterial taxa in the gut microbiome was assessed using 16S rRNA analysis. Fusobacterium nucleatum was the only bacterium that was significantly (P < 0.0001) more prevalent in the cancer tissue (82.1%) than in the normal tissue (0%) by qPCR. 16S rRNA analysis showed a significant correlation between six operational taxonomic units (OTUs), namely, the genera Fusobacterium, Peptostreptococcus, Collinsella, Prevotella, Parvimonas, and Gemella, in patients with CRC. An integrated analysis using 16S rRNA data and epidemiological characteristics showed that alcohol consumption was significantly correlated with the abundance of Fusobacterium OTUs. The correlation of alcohol consumption with the abundance of Fusobacterium OTUs in cancer tissue discovered using 16S rRNA analysis suggests a possible link between alcohol metabolism and subsequent tumorigenesis caused by F. nucleatum.
个体细菌的作用及其在结直肠癌（CRC）发展中的关系尚不清楚。我们旨在使用定量实时 PCR（qPCR）或 16S rRNA 分析来确定 CRC 相关细菌的流行率，并确定患者人口统计学和临床特征（包括饮酒）与 CRC 相关细菌的统计相关性。我们使用 qPCR 确定了 38 名 CRC 患者（39 个样本）和 21 名正常个体中五种 CRC 相关细菌的流行率，并使用 16S rRNA 分析评估了肠道微生物组中细菌分类群的相对丰度。qPCR 结果显示，具核梭杆菌是唯一一种在癌症组织（82.1%）中比正常组织（0%）更普遍存在的细菌（P<0.0001）。16S rRNA 分析显示，CRC 患者中有六个分类单元（OTU），即梭杆菌属、消化链球菌属、柯林斯氏菌属、普雷沃氏菌属、卟啉单胞菌属和孪生球菌属之间存在显著相关性。使用 16S rRNA 数据和流行病学特征的综合分析表明，饮酒与梭杆菌属 OTU 的丰度显著相关。16S rRNA 分析显示，饮酒与癌症组织中梭杆菌属 OTU 的丰度相关，这表明酒精代谢与具核梭杆菌引起的随后肿瘤发生之间可能存在联系。
