Faculty of Pharmacy, Gomal University, D. I. Khan, Pakistan.
Department of Biotechnology and Bioinformatics, International Islamic University Islamabad, Islamabad, Pakistan.
AAPS PharmSciTech. 2020 Nov 16;21(8):323. doi: 10.1208/s12249-020-01866-y.
Crude ginger has been used to treat wounds since ancient times till nowadays. The present study aimed at designing and characterizing topical hydrogel films loaded with ginger extract for wound healing in animal model. The hydrogel films were prepared using PVA and gelatin. The prepared films were evaluated for FTIR analysis, surface morphology, pH, swelling behavior, in vitro release, and % drug content. The wound-healing activity of the extract-loaded hydrogel films was compared with commercially available Silver Sulfadiazine® cream. The drug was compatible with the selected polymers and indicated the suitability of the selected polymers for preparation of topical hydrogel films. The SEM images clearly indicated porous structure of the prepared hydrogel films. Slight changes were observed in pH, ranging from 4.98 ± 0.079 in the beginning of the study to 4.9 ± 0.58 in the end. The swelling percentage after 8 h was 257.7%. The films released 78.7 ± 1.7% of the drug in 250 min. The percent drug content was 97.78 ± 5% which did not change significantly during the storage period. The hydrogel films showed similar wound-healing activity as compared to the commercial product (p > 0.05; ANOVA), while greater wound-healing activity as compared to the control group (p < 0.05; ANOVA) evidenced by intensive collagen formation in histopathological analysis.
生姜自古以来就被用于治疗伤口。本研究旨在设计并表征负载生姜提取物的局部水凝胶薄膜，用于动物模型中的伤口愈合。水凝胶薄膜是使用 PVA 和明胶制备的。对制备的薄膜进行傅里叶变换红外光谱（FTIR）分析、表面形貌、pH 值、溶胀行为、体外释放和药物含量%的评估。将载药水凝胶薄膜的伤口愈合活性与市售磺胺嘧啶银乳膏进行比较。药物与所选聚合物相容，表明所选聚合物适合制备局部水凝胶薄膜。SEM 图像清楚地表明了制备的水凝胶薄膜的多孔结构。在研究开始时 pH 值从 4.98 ± 0.079 略有变化，在研究结束时 pH 值为 4.9 ± 0.58。8 小时后的溶胀率为 257.7%。在 250 分钟内，薄膜释放了 78.7 ± 1.7%的药物。药物含量%为 97.78 ± 5%，在储存期间没有明显变化。水凝胶薄膜的伤口愈合活性与商业产品相似（p>0.05；ANOVA），而与对照组相比，水凝胶薄膜的伤口愈合活性更高（p<0.05；ANOVA），组织病理学分析表明胶原蛋白形成更密集。
