Department of Biomedical Chemistry and Polymer Science, Medicinal Chemistry Research Group, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100, Torun, Poland.
Department of Medicinal Chemistry, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum, Jurasza 2, 85-089, Bydgoszcz, Poland.
Sci Rep. 2022 Nov 4;12(1):18658. doi: 10.1038/s41598-022-23506-0.
This study focuses on obtaining and characterizing novel chitosan-based biomaterials containing cannabis oil to potentially promote wound healing. The primary active substance in cannabis oil is the non-psychoactive cannabidiol, which has many beneficial properties. In this study, three chitosan-based films containing different concentrations of cannabis oil were prepared. As the amount of oil increased, the obtained biomaterials became rougher as tested by atomic force microscopy. Such rough surfaces promote protein adsorption, confirmed by experiments assessing the interaction between human albumin with the obtained materials. Increased oil concentration also improved the films' mechanical parameters, swelling capacity, and hydrophilic properties, which were checked by the wetting angle measurement. On the other hand, higher oil content resulted in decreased water vapour permeability, which is essential in wound dressing. Furthermore, the prepared films were subjected to an acute toxicity test using a Microtox. Significantly, the film's increased cannabis oil content enhanced the antimicrobial effect against A. fischeri for films in direct contact with bacteria. More importantly, cell culture studies revealed that the obtained materials are biocompatible and, therefore, they might be potential candidates for application in wound dressing materials.
本研究专注于获取和表征新型壳聚糖基生物材料，其中含有大麻油，以潜在促进伤口愈合。大麻油中的主要活性物质是非精神活性大麻二酚，它具有许多有益的特性。在这项研究中，制备了三种含有不同浓度大麻油的壳聚糖基薄膜。随着油含量的增加，通过原子力显微镜测试，所获得的生物材料变得更加粗糙。这种粗糙的表面促进了蛋白质的吸附，通过评估人白蛋白与所获得的材料之间的相互作用的实验得到了证实。油浓度的增加还改善了薄膜的机械性能参数、溶胀能力和亲水性能，通过润湿角测量进行了检查。另一方面，较高的油含量导致水蒸气渗透率降低，这在伤口敷料中是必不可少的。此外，还使用 Microtox 对所制备的薄膜进行了急性毒性测试。值得注意的是，随着薄膜中大麻油含量的增加，直接与细菌接触的薄膜对发光菌 A. fischeri 的抗菌效果增强。更重要的是，细胞培养研究表明，所获得的材料具有生物相容性，因此它们可能是伤口敷料材料应用的潜在候选材料。
