Department of Genetic Engineering, Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Acad.Zabolotnoho str, 148, Kyiv, 03143, Ukraine.
Zhytomyr Ivan Franko State University, Zhytomyr, Ukraine.
Appl Biochem Biotechnol. 2021 Apr;193(4):1215-1230. doi: 10.1007/s12010-020-03471-y. Epub 2020 Nov 17.
This study evaluated the potential of dwarf morning-glory Convolvulus tricolor (Convolvulaceae) plants and their hairy roots induced by Agrobacterium rhizogenes for rhizoextraction of heavy metals ions from the liquid medium under aseptic growth conditions. Both the young C. tricolor plants and the generated hairy root culture efficiently removed Cr, Ni, and Pb ions from the liquid cultivation medium. As determined by atomic absorption spectroscopy, the hairy roots demonstrated a high level of heavy metal ions accumulation (μg/g dry weight): 3942 ± 210 of chromium, 1529 ± 312 of nickel, and 2613 ± 373 of lead. These data show that the hairy roots of morning glory might be of interest for some phytoremediation strategies due to their high bioaccumulation abilities. The comparison of bioaccumulation potential of established hairy roots and young C. tricolor plants give grounds to suppose that roots of C. tricolor play an active role in the absorption of Cr, Ni, and Pb from liquid media, whereas the aboveground part rather serves as a storage for the accumulated metal ions.
本研究评估了矮牵牛 Convolvulus tricolor（旋花科）植株及其发根农杆菌诱导的毛状根在无菌生长条件下从液体培养基中提取重金属离子的潜力。在液体培养中，无论是年轻的 C. tricolor 植株还是生成的毛状根培养物，都能有效地去除 Cr、Ni 和 Pb 离子。原子吸收光谱法测定，毛状根表现出高水平的重金属离子积累（μg/g 干重）：3942±210 铬、1529±312 镍和 2613±373 铅。这些数据表明，由于具有较高的生物累积能力，长春花的毛状根可能对某些植物修复策略具有吸引力。已建立的毛状根和年轻的 C. tricolor 植物的生物累积潜力的比较表明，C. tricolor 的根在从液体培养基中吸收 Cr、Ni 和 Pb 方面发挥积极作用，而地上部分则更像是积累金属离子的储存库。
