Graduation Program in Chemistry, University of Blumenau, Rua Antonio da Veiga 140, 89030-903, Blumenau, SC, Brazil.
School of Biomedical Sciences, University of Blumenau, Rua São Paulo 2171, Campus 3, 89030-001, Blumenau, SC, Brazil.
Chem Biodivers. 2021 Jan;18(1):e2000711. doi: 10.1002/cbdv.202000711. Epub 2020 Dec 7.
Hydroalcoholic propolis extracts from the bee species Melipona quadrifasciata have been shown to possess antimicrobial activity against different mollicute strains, but a methanolic extract (ME) could contain an increased diversity of nonpolar bioactive components with a potentially higher antimicrobial activity. The ME obtained by maceration of the propolis sample was fractionated with solvents of different polarities and then, purified by silica gel column chromatography through biomonitoring of its antimicrobial activity against mollicute strains. Analysis by gas chromatography-mass spectrometry (GC/MS) enabled the identification of compounds using the NIST library. Minimum inhibitory concentrations (MICs) of the samples were determined by broth microdilution. Anti-adhesive assays were performed with Mycoplasma pneumoniae cells. The hexane (MIC=62.5 mg/L) and dichloromethane (MIC=125 mg/L) fractions presented the most promising results against M. pneumoniae. They were fractionated into 74 subfractions, and even the best ones did not show better results (MIC>250 mg/L) than their original fractions, likely due to the loss of terpene compounds that seem to act in synergy. The dichloromethane subfraction FD4 was highlighted in the anti-adhesive assay with an inhibitory activity of 21.6 %. A synergistic effect of the nonpolar compounds in M. quadrifasciata propolis may be responsible for its antibacterial activity, but several purified components can improve its anti-adhesive properties.
从蜜蜂物种 Melipona quadrifasciata 中提取的水醇蜂胶提取物已被证明具有抗不同柔膜体菌株的抗菌活性，但甲醇提取物（ME）可能含有更多种类的非极性生物活性成分，具有更高的抗菌活性。通过浸渍法从蜂胶样品中获得的 ME 用不同极性的溶剂进行了分级，然后通过硅胶柱色谱法对其进行了纯化，通过监测其对柔膜体菌株的抗菌活性来进行生物监测。通过气相色谱-质谱联用分析（GC/MS），使用 NIST 库对化合物进行了分析。通过肉汤微量稀释法测定了样品的最小抑菌浓度（MIC）。采用肺炎支原体细胞进行抗粘附试验。正己烷（MIC=62.5mg/L）和二氯甲烷（MIC=125mg/L）馏分对肺炎支原体表现出最有希望的结果。它们被进一步分成 74 个亚馏分，即使是最好的亚馏分也没有比原始馏分表现出更好的结果（MIC>250mg/L），这可能是由于萜类化合物的损失，这些化合物似乎协同作用。二氯甲烷亚馏分 FD4 在抗粘附试验中表现出 21.6%的抑制活性。非极性化合物在 M. quadrifasciata 蜂胶中的协同作用可能是其抗菌活性的原因，但几种纯化成分可以改善其抗粘附特性。
