Federal University of Alfenas, CEP, MG-37130-001, Alfenas, Brazil.
Luiz de Queiroz College of Agriculture, University of São Paulo, CEP, SP-13418-900, Piracicaba, Brazil.
Chem Biodivers. 2024 Jun;21(6):e202302084. doi: 10.1002/cbdv.202302084. Epub 2024 May 17.
This study explores the potential of propolis, a resinous substance produced by bees, from Melipona rufiventris species. With its composition encompassing resin, wax, pollen, and soil, propolis holds historical significance in traditional medicine within tropical regions. This research is driven by the scarcity of information surrounding M. rufiventris propolis, prompting an investigation into its chemical constituents, in vivo toxicity, and antimicrobial, antioxidant, and anti-inflammatory properties. This exploration could potentially uncover novel applications for this natural product, bolstering both meliponiculture practices and the preservation of native bee populations. The propolis was sampled in Cabo Verde-MG and underwent ethanolic extraction to yield an extract (EEP) for analysis. Chemical assessments (Folin-Ciocalteau, and UHPLC-HRMS) revealed the presence of polyphenols, including flavonoids. The EEP demonstrated higher antimicrobial activity against Gram-positive bacteria and exhibited efficacy against multiresistant strains isolated from complex wounds. Synergistic interactions with commercial antibiotics were also observed. Furthermore, anti-inflammatory evaluations showcased the EEP's potential in reducing NF-kB activation and TNF-α release at non-toxic concentrations. Despite these promising biological activities, the EEP exhibited no antiproliferative effects and demonstrated safety in both the MTS assay and the G. mellonella model. Collectively, these findings highlight the M. rufiventris propolis extract as a valuable reservoir of bioactive compounds with multifaceted potential.
这项研究探索了产自 Melipona rufiventris 物种的蜂胶的潜力，蜂胶是一种由蜜蜂产生的树脂状物质。由于其组成包括树脂、蜡、花粉和土壤，蜂胶在热带地区的传统医学中具有历史意义。这项研究的驱动力是围绕 M. rufiventris 蜂胶的信息稀缺，促使人们调查其化学成分、体内毒性以及抗菌、抗氧化和抗炎特性。这一探索有可能为这种天然产物开辟新的应用，同时增强蜜蜂养殖实践和保护本地蜜蜂种群。蜂胶在巴西的卡博弗里乌-米纳斯吉拉斯州进行了采样，并进行了乙醇提取以获得提取物（EEP）进行分析。化学评估（福林-肖卡尔特法和 UHPLC-HRMS）表明存在多酚，包括类黄酮。EEP 对革兰氏阳性菌表现出更高的抗菌活性，并对从复杂伤口中分离出的多药耐药菌株有效。还观察到与商业抗生素的协同相互作用。此外，抗炎评估表明 EEP 具有在非毒性浓度下降低 NF-kB 激活和 TNF-α释放的潜力。尽管具有这些有希望的生物学活性，但 EEP 在 MTS 测定和 G. mellonella 模型中均未显示出抗增殖作用且安全。总的来说，这些发现强调了 M. rufiventris 蜂胶提取物是一种具有多方面潜力的生物活性化合物的宝贵来源。
