International Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun, 130118, P. R. China.
Guizhou Academy of Agricultural Sciences, Guizhou Key Laboratory of Edible fungi breeding, Guiyang, 550006, P. R. China.
J Microbiol. 2021 Jan;59(1):41-50. doi: 10.1007/s12275-021-0242-5. Epub 2020 Nov 17.
Gloeostereum incarnatum is an edible medicinal mushroom widely grown in China. Using the whole genome of G. incarnatum, simple sequence repeat (SSR) markers were developed and synthetic primers were designed to construct its first genetic linkage map. The 1,048.6 cm map is composed of 10 linkage groups and contains 183 SSR markers. In total, 112 genome assembly sequences were anchored, representing 16.43 Mb and covering 46.41% of the genome. Selfing populations were used for quantitative trait loci (QTL) targeting, and the composite interval mapping method was used to co-localize the mycelium growth rate (potato dextrose agar and sawdust), growth period, yield and fruiting body length, and width and thickness. The 14 QTLs of agronomic traits had LOD values of 3.20-6.51 and contribution rates of 2.22-13.18%. No linkage relationship was found between the mycelium growth rate and the growth period, but a linkage relationship was observed among the length, width and thickness of the fruiting bodies. Using NCBI's BLAST alignment, the genomic sequences corresponding to the QTL regions were compared, and a TPR-like protein candidate gene was selected. Using whole-genome data, 138 candidate genes were found in four sequence fragments of two SSR markers located in the same scaffold. The genetic map and QTLs established in this study will aid in developing selective markers for agronomic traits and identifying corresponding genes, thereby providing a scientific basis for the further gene mapping of quantitative traits and the marker-assisted selection of functional genes in G. incarnatum breeding programs.
蝉花是一种在中国广泛种植的可食用药用真菌。利用蝉花的全基因组，开发了简单序列重复（SSR）标记，并设计了合成引物来构建其第一张遗传连锁图谱。该图谱长 1048.6cm，由 10 个连锁群组成，包含 183 个 SSR 标记。总共锚定了 112 个基因组组装序列，代表 16.43Mb，覆盖基因组的 46.41%。利用自交群体进行数量性状位点（QTL）定位，采用复合区间作图法共定位了菌丝生长速度（马铃薯葡萄糖琼脂和木屑）、生长周期、产量和子实体长度、宽度和厚度。14 个农艺性状 QTL 的 LOD 值为 3.20-6.51，贡献率为 2.22-13.18%。菌丝生长速度与生长周期之间没有发现连锁关系，但在子实体的长度、宽度和厚度之间存在连锁关系。利用 NCBI 的 BLAST 比对，对 QTL 区域的基因组序列进行了比较，选择了一个 TPR 样蛋白候选基因。利用全基因组数据，在位于同一支架上的两个 SSR 标记的四个序列片段中发现了 138 个候选基因。本研究建立的遗传图谱和 QTL 将有助于开发农艺性状的选择标记和鉴定相应的基因，为进一步进行蝉花子实体数量性状的基因图谱定位和功能基因的标记辅助选择提供科学依据。
