Lu Li-Xin, Yao Fang-Jie, Wang Peng, Fang Ming, Zhang You-Min, Zhang Wei-Tong, Kong Xiang-Hui, Lu Jia
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, P. R. China.
College of Horticulture, Jilin Agricultural University, Changchun, 130118, P. R. China.
J Microbiol. 2017 Oct;55(10):792-799. doi: 10.1007/s12275-017-7241-6. Epub 2017 Sep 28.
Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A. auricula-judae and amplified in A14-5, A18- 119, and the mapping population. The map consisted of 11 linkage groups (LGs) spanning 854 cM, with an average interval length of 6.57 cM. A testcross population was derived from crossing between the monokaryon A184-57 (from the wild strain A184 as a tester strain) and the mapping population. Important agronomic trait-related QTLs, including mycelium growth rate on potato dextrose agar for the mapping population, mycelium growth rate on potato dextrose agar and sawdust for the testcross population, growth period (days from inoculation to fruiting body harvesting), and yield for the testcross population, were identified using the composite interval mapping method. Six mycelium growth raterelated QTLs were identified on LG1 and LG4, two growth period-related QTLs were identified on LG2, and three yieldrelated QTLs were identified on LG2 and LG6. The results showed no linkage relationship between mycelium growth rate and growth period. The present study provides a foundation for locating genes for important agronomic characteristics in A. auricula-judae in the future.
黑木耳是一种在中国广泛种植的传统食用菌。在本研究中，利用由138个来自杂交菌株（A119 - 5）的单核体组成的作图群体构建了黑木耳的遗传连锁图谱。单核体亲本菌株A14 - 5和A18 - 119分别来自两个栽培品种A14（齐黑1号）和A18（齐黑2号）。总共定位了130个简单序列重复标记。这些标记是利用黑木耳的全基因组序列开发的，并在A14 - 5、A18 - 119和作图群体中进行扩增。该图谱由11个连锁群（LGs）组成，跨度为854厘摩，平均间隔长度为6.57厘摩。一个测交群体是通过单核体A184 - 57（来自野生菌株A184作为测试菌株）与作图群体杂交得到的。利用复合区间作图法鉴定了重要的农艺性状相关QTL，包括作图群体在马铃薯葡萄糖琼脂上的菌丝生长速率、测交群体在马铃薯葡萄糖琼脂和木屑上的菌丝生长速率、生育期（从接种到子实体采收的天数）以及测交群体的产量。在LG1和LG4上鉴定出6个与菌丝生长速率相关的QTL，在LG2上鉴定出2个与生育期相关的QTL，在LG2和LG6上鉴定出3个与产量相关的QTL。结果表明菌丝生长速率与生育期之间没有连锁关系。本研究为未来定位黑木耳重要农艺性状的基因奠定了基础。
