Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China.
Department of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Genes (Basel). 2019 Mar 1;10(3):188. doi: 10.3390/genes10030188.
is a precious edible mushroom that is widely grown in Asia and known for its useful medicinal properties. Here, we present a high-quality genome of using the single-molecule real-time (SMRT) sequencing platform. The genome, which is the first complete genome to be sequenced in the family , was 38.67 Mbp, with an N50 of 3.5 Mbp, encoding 15,251 proteins. Based on our phylogenetic analysis, the diverged ~174 million years ago. Several genes and gene clusters associated with lignocellulose degradation, secondary metabolites, and polysaccharide biosynthesis were identified in , and compared with other medicinal mushrooms. In particular, we identified two terpenoid-associated gene clusters, each containing a gene encoding a sesterterpenoid synthase adjacent to a gene encoding a cytochrome P450 enzyme. These clusters might participate in the biosynthesis of incarnal, a known bioactive sesterterpenoid produced by . Through a transcriptomic analysis comparing the mycelium and fruiting body, we also demonstrated that the genes associated with terpenoid biosynthesis were generally upregulated in the mycelium, while those associated with polysaccharide biosynthesis were generally upregulated in the fruiting body. This study provides insights into the genetic basis of the medicinal properties of laying a framework for future characterization of bioactive proteins and pharmaceutical uses of this fungus.
是一种珍贵的食用蘑菇，广泛生长在亚洲，因其具有有用的药用特性而闻名。在这里，我们使用单分子实时 (SMRT) 测序平台呈现了 的高质量基因组。 基因组是该家族中第一个被测序的完整基因组，大小为 38.67 Mbp，N50 为 3.5 Mbp，编码 15251 种蛋白质。根据我们的系统发育分析， 大约在 1.74 亿年前分化而来。在 中鉴定到了几个与木质纤维素降解、次生代谢物和多糖生物合成相关的基因和基因簇，并与其他药用蘑菇进行了比较。特别是，我们鉴定出了两个萜类化合物相关的基因簇，每个基因簇都包含一个编码倍半萜合酶的基因，其紧邻一个编码细胞色素 P450 酶的基因。这些簇可能参与了 incarnal 的生物合成，incarnal 是一种已知的由 产生的生物活性倍半萜。通过比较 菌丝体和子实体的转录组分析，我们还表明，与萜类化合物生物合成相关的基因在菌丝体中通常上调，而与多糖生物合成相关的基因在子实体中通常上调。这项研究为 提供了对药用特性的遗传基础的深入了解，为进一步研究该真菌的生物活性蛋白和药用用途奠定了框架。
