Jiang Wan-Zhu, Yao Fang-Jie, Fang Ming, Lu Li-Xin, Zhang You-Min, Wang Peng, Meng Jing-Jing, Lu Jia, Ma Xiao-Xu, He Qi, Shao Kai-Sheng, Khan Asif Ali, Wei Yun-Hui
International Cooperation Research Center of China for New Germplasm Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun, China.
College of Horticulture, Jilin Agricultural University, Changchun, China.
Mycobiology. 2021 Aug 12;49(4):406-420. doi: 10.1080/12298093.2021.1954321. eCollection 2021.
has edible and medicinal value and was first cultivated and domesticated in China. We sequenced the monokaryotic strain GiC-126 on an Illumina HiSeq X Ten system and obtained a 34.52-Mb genome assembly sequence that encoded 16,895 predicted genes. We combined the GiC-126 genome with the published genome of strain CCMJ2665 to construct a genetic linkage map (GiC-126 genome) that had 10 linkage groups (LGs), and the 15 assembly sequences of CCMJ2665 were integrated into 8 LGs. We identified 1912 simple sequence repeat (SSR) loci and detected 700 genes containing 768 SSRs in the genome; 65 and 100 of them were annotated with gene ontology (GO) terms and KEGG pathways, respectively. Carbohydrate-active enzymes (CAZymes) were identified in 20 fungal genomes and annotated; among them, 144 CAZymes were annotated in the GiC-126 genome. The A mating-type locus () of was located on scaffold885 at 38.9 cM of LG1 and was flanked by two homeodomain (HD1) genes, and . Fourteen segregation distortion markers were detected in the genetic linkage map, all of which were skewed toward the parent GiC-126. They formed three segregation distortion regions (SDR1-SDR3), and 22 predictive genes were found in scaffold1920 where three segregation distortion markers were located in SDR1. In this study, we corrected and updated the genomic information of . Our results will provide a theoretical basis for fine gene mapping, functional gene cloning, and genetic breeding the follow-up of .
具有食用和药用价值，最早在中国被栽培和驯化。我们在Illumina HiSeq X Ten系统上对单核菌株GiC-126进行了测序，获得了一个34.52 Mb的基因组组装序列，该序列编码16,895个预测基因。我们将GiC-126基因组与已发表的菌株CCMJ2665的基因组相结合，构建了一个具有10个连锁群（LGs）的遗传连锁图谱（GiC-126基因组），CCMJ2665的15个组装序列被整合到8个LGs中。我们在基因组中鉴定出1912个简单序列重复（SSR）位点，并检测到700个包含768个SSR的基因；其中65个和100个分别用基因本体论（GO）术语和KEGG途径进行了注释。在20个真菌基因组中鉴定并注释了碳水化合物活性酶（CAZymes）；其中，在GiC-126基因组中注释了144个CAZymes。其A交配型位点位于LG1的38.9 cM处的scaffold885上，两侧是两个同源结构域（HD1）基因，即 和 。在遗传连锁图谱中检测到14个分离畸变标记，所有这些标记都偏向亲本GiC-126。它们形成了三个分离畸变区域（SDR1-SDR3），在SDR1中位于三个分离畸变标记所在的scaffold1920中发现了22个预测基因。在本研究中，我们校正并更新了 的基因组信息。我们的结果将为后续 的精细基因定位、功能基因克隆和遗传育种提供理论依据。
