Ji Yuan Yuan, Meng Man, Miao Ye
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People's Republic of China.
Department of Geriatrics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People's Republic of China.
Cancer Manag Res. 2020 Nov 9;12:11423-11433. doi: 10.2147/CMAR.S277064. eCollection 2020.
Cervical cancer is a common gynecologic cancer, and no study has been reported on the way through which lncRNA SNHG1, miR-195 and NEK2 jointly affect cervical cancer cells (CCCs), so this paper will explore a new approach to the development of cervical cancer in this respect.
Altogether 72 cervical cancer tissues and 54 adjacent tissues were collected. qPCR was performed to quantify lncRNA SNHG1 and miR-195, whose expression vectors were constructed and then transfected into CCCs, so as to observe their effects on the cells. Western blotting (WB) was carried out to detect protein levels. MTT assay was conducted to detect cell activity. Flow cytometry was performed to detect cell apoptosis. Transwell was carried out to detect cell invasion and migration.
The expression of lncRNA SNHG1 up-regulated while that of miR-195 down-regulated in CCCs. lncRNA SNHG1 regulated NEK2 through its targeted binding to miR-195. The down-regulation of lncRNA SNHG1 or the up-regulation of miR-195 led to the decrease of NEK2 and the reduction of cells' activity, migration and invasion, also resulting in the increase of cell apoptosis. Rescue experiments showed that the down-regulation of miR-195 could offset the cell changes caused by lncRNA SNHG1.
lncRNA SNHG1 promotes the progression of cervical cancer through the miR-195/NEK2 axis, so lncRNA SNHG1, miR-195 and NEK2 may have potential values for diagnosing and treating cervical cancer.
宫颈癌是一种常见的妇科癌症，目前尚无关于长链非编码RNA SNHG1、微小RNA-195（miR-195）和NIMA相关激酶2（NEK2）联合影响宫颈癌细胞（CCC）途径的研究报道，因此本文将在这方面探索一种宫颈癌发生发展的新途径。
共收集72例宫颈癌组织和54例癌旁组织。采用实时荧光定量聚合酶链反应（qPCR）对lncRNA SNHG1和miR-195进行定量分析，构建其表达载体后转染至CCC中，观察其对细胞的影响。采用蛋白质免疫印迹法（WB）检测蛋白水平。采用噻唑蓝（MTT）比色法检测细胞活性。采用流式细胞术检测细胞凋亡。采用Transwell小室实验检测细胞侵袭和迁移能力。
lncRNA SNHG1在CCC中的表达上调，而miR-195的表达下调。lncRNA SNHG1通过靶向结合miR-195调控NEK2。lncRNA SNHG1表达下调或miR-195表达上调导致NEK2减少，细胞活性、迁移和侵袭能力降低，同时细胞凋亡增加。挽救实验表明，miR-195表达下调可抵消lncRNA SNHG1引起的细胞变化。
lncRNA SNHG1通过miR-195/NEK2轴促进宫颈癌进展，因此lncRNA SNHG1、miR-195和NEK2在宫颈癌的诊断和治疗中可能具有潜在价值。
