Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China.
Biomed Res Int. 2020 Nov 2;2020:2420437. doi: 10.1155/2020/2420437. eCollection 2020.
Ribosome biogenesis regulatory protein homolog (RRS1) is a protein required for ribosome biogenesis. Recent studies have identified an oncogenic role of RRS1 in some cancers, whereas the involvement of RRS1 in retinoblastoma (RB) remains to be determined. In this study, we aimed to explore the role of RRS1 in RB. We found that the expression of RRS1 was increased in RB tissues and cells. Lentivirus-mediated RRS1 overexpression promoted the proliferation, growth, and invasion of RB cells. Opposite results were found in RRS1 knockdown cells. In addition, RRS1 silencing induced cell cycle arrest at the G1 phase and apoptosis in RB cells, while RRS1 ectopic expression exhibited the opposite effect. At the molecular level, RRS1 activated the AKT/mTOR signaling pathway, inhibition of which largely blunted the proliferation, growth, and invasion of RB cells. Our study suggests that RRS1 functions as an oncogene in RB through activating the AKT/mTOR signaling pathway.
核糖体生物发生调节蛋白同源物（RRS1）是一种核糖体生物发生所必需的蛋白质。最近的研究表明，RRS1 在某些癌症中具有致癌作用，而 RRS1 在视网膜母细胞瘤（RB）中的参与仍有待确定。在这项研究中，我们旨在探讨 RRS1 在 RB 中的作用。我们发现 RRS1 的表达在 RB 组织和细胞中增加。慢病毒介导的 RRS1 过表达促进了 RB 细胞的增殖、生长和侵袭。在 RRS1 敲低细胞中则发现了相反的结果。此外，RRS1 沉默诱导 RB 细胞周期停滞在 G1 期并促进细胞凋亡，而 RRS1 异位表达则表现出相反的效果。在分子水平上，RRS1 激活了 AKT/mTOR 信号通路，该通路的抑制在很大程度上削弱了 RB 细胞的增殖、生长和侵袭。我们的研究表明，RRS1 通过激活 AKT/mTOR 信号通路在 RB 中发挥致癌基因的作用。
