Department of Dermatology, College of Medicine, Eunpyeong St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
Mycoses. 2021 Mar;64(3):292-299. doi: 10.1111/myc.13214. Epub 2020 Dec 2.
Malassezia species are associated with several common dermatologic conditions including pityriasis versicolor, seborrhoeic dermatitis, folliculitis, and atopic dermatitis and dandruff. However, its causal role remains to be established. We intended to explore the role of inflammasome activation in human keratinocytes in response to three different Malassezia species. We compared the different activation patterns of inflammasomes and the expression of pro-inflammatory cytokines and antimicrobial peptides by three different Malassezia species-M. restricta, M. globosa and M. sympodialis-in human keratinocytes. We found that different Malassezia species, especially M. restricta and M. globosa could induce nucleotide-binding oligomerisation domain, leucine-rich repeat and pyrin-domain-containing protein (NLRP)3-apoptosis-associated speck-like protein containing CARD (ASC) inflammasome activation and subsequent interleukin (IL)-1β secretion in human keratinocytes. Malassezia species variably induced thymic stromal lymphopoietin, β-defensin 2, and LL-37. IL-8 mRNA and IL-22 protein significantly increased in the M. sympodialis-treated group, and Chemokine C-C motif ligand (CCL)17 and CCL22 mRNA were increased in response to M. globosa- and M. restricta- treated keratinocytes, respectively. Our data show that various species of Malassezia promote variable inflammatory responses in keratinocytes by activating NLRP3 inflammasomes, pro-inflammatory cytokines and chemokines, and antimicrobial peptides.
马拉色菌属与几种常见的皮肤科疾病有关，包括花斑癣、脂溢性皮炎、毛囊炎和特应性皮炎以及头皮屑。然而，其因果关系仍有待确定。我们旨在探索炎症小体激活在人类角质形成细胞中对三种不同马拉色菌的作用。我们比较了三种不同的马拉色菌——限制马拉色菌、球形马拉色菌和合轴马拉色菌——在人类角质形成细胞中激活炎症小体和表达促炎细胞因子和抗菌肽的不同模式。我们发现，不同的马拉色菌，特别是限制马拉色菌和球形马拉色菌，能够诱导核苷酸结合寡聚化结构域、富含亮氨酸重复和吡咯烷结构域包含蛋白（NLRP）3-凋亡相关斑点样蛋白包含 CARD（ASC）炎症小体激活，并随后诱导人角质形成细胞分泌白细胞介素（IL）-1β。马拉色菌属的不同物种可诱导胸腺基质淋巴细胞生成素、β-防御素 2 和 LL-37。在 M. sympodialis 处理组中，IL-8 mRNA 和 IL-22 蛋白显著增加，而对 M. globosa 和 M. restricta 处理的角质形成细胞，趋化因子 C-C 基序配体（CCL）17 和 CCL22 mRNA 增加。我们的数据表明，各种马拉色菌通过激活 NLRP3 炎症小体、促炎细胞因子和趋化因子以及抗菌肽，促进角质形成细胞中不同的炎症反应。
