Monteiro Fernanda R, Roseira Tamaris, Amaral Jonatas B, Paixão Vitória, Almeida Ewin B, Foster Roberta, Sperandio Adriane, Rossi Marcelo, Amirato Gislene R, Apostólico Juliana S, Santos Carlos A F, Felismino Eduardo S, Leal Fabyano B, Thomazelli Luciano M, Durigon Edison L, Oliveira Danielle B L, Vieira Rodolfo P, Santos Juliana M B, Bachi André L L
Department of Otorhinolaryngology, ENT Lab, Federal University of Sao Paulo (UNIFESP), Sao Paulo CEP 04025-002, Brazil.
Method Faculty of Sao Paulo (FAMESP), Sao Paulo CEP 04046-200, Brazil.
Vaccines (Basel). 2020 Nov 16;8(4):685. doi: 10.3390/vaccines8040685.
Since aging affects the immune responses against vaccination, the present study evaluated the effects of L-glutamine (Gln) supplementation in the humoral and cellular immune responses in elderly subjects, practitioners or not, of physical exercise training.
Eighty-four elderly people (aged 72.6 ± 6.1), non-practitioners (NP, = 31), and practitioners of combined-exercise training (CET, = 53) were submitted to Influenza virus vaccination and supplemented with Gln (0.3 g/kg of weight + 10 g of maltodextrin, groups: NP-Gln ( = 14), and CET-Gln ( = 26)), or placebo (10 g of maltodextrin, groups: NP-PL ( = 17), and CET-PL ( = 27)). Blood samples were collected pre (baseline) and 30 days post-vaccination and supplementation.
Comparing with the baseline values, whereas the NP-Gln and CET-PL groups showed higher specific-IgM levels, the CET-Gln group showed higher specific-IgM and IgA levels post-vaccination. The titer rate of hemagglutination inhibition was higher in the CET-Gln, NP-PL, and NP-Gln groups post-vaccination than baseline values. The absolute number of naive and effector CD4+ T cells was higher especially in the NP-Gln and CET-Gln groups, whilst activated CD4+ T cells were higher in CET subgroups post-vaccination.
Our results showed that both l-glutamine supplementation and combined-exercise training can improve the immune responses to the Influenza virus vaccine in elderly subjects.
由于衰老会影响针对疫苗接种的免疫反应，本研究评估了补充L-谷氨酰胺（Gln）对老年体育锻炼参与者和非参与者体液免疫和细胞免疫反应的影响。
84名老年人（年龄72.6±6.1岁），非体育锻炼参与者（NP，n = 31）和联合运动训练参与者（CET，n = 53）接种流感病毒疫苗，并补充Gln（0.3 g/kg体重+ 10 g麦芽糊精，组：NP-Gln（n = 14）和CET-Gln（n = 26））或安慰剂（10 g麦芽糊精，组：NP-PL（n = 17）和CET-PL（n = 27））。在接种疫苗和补充前（基线）以及接种后30天采集血样。
与基线值相比，NP-Gln组和CET-PL组的特异性IgM水平较高，而CET-Gln组在接种疫苗后特异性IgM和IgA水平较高。接种疫苗后，CET-Gln组、NP-PL组和NP-Gln组的血凝抑制滴度率高于基线值。尤其是NP-Gln组和CET-Gln组中幼稚和效应CD4+ T细胞的绝对数量较高，而接种疫苗后CET亚组中活化CD4+ T细胞数量较高。
我们的结果表明，补充L-谷氨酰胺和联合运动训练均可改善老年受试者对流感病毒疫苗的免疫反应。
