Department of Biotechnology and Research, KVM College of Engineering and IT, Alappuzha, Kerala, India.
Department of Microbiology, Central Coir Research Institute (CCRI), Alappuzha, Kerala, India.
Nat Prod Res. 2022 Feb;36(4):901-908. doi: 10.1080/14786419.2020.1849194. Epub 2020 Nov 19.
Coir pith, an agro-industrial residue, is resistant to natural degradation, and its accumulation causes environmental pollution. Ferulic acid, a precursor of vanillin, was extracted from the raw coir pith by chemical pre-treatment such as alkaline hydrolysis, acidification, and liquid-liquid extraction method. The obtained ferulic acid (1.2 g/50 g) was analysed using high-performance liquid chromatography (HPLC) and used as a substrate for biotransformation by gg to vanillic acid, which, in turn, was fermented by using to vanillin. The quantity of vanillic acid detected by HPLC on the third day of incubation was 0.773 g/L, while the optimal yield of vanillin on the subsequent third day of incubation was 0.628 g/L. Thus, the chemical extraction of ferulic acid from coir pith ensued bioconversion into vanillin. These products are highly valuable and economical to be used in industries such as pharmaceuticals, health, cosmetics, and neutraceuticals.
椰糠纤维是一种农业工业废物，它难以自然降解，其堆积会造成环境污染。阿魏酸是香草醛的前体物质，可以通过碱水解、酸化和液-液萃取等化学预处理方法从椰糠纤维原料中提取。用高效液相色谱法（HPLC）分析得到的阿魏酸（1.2g/50g），并用 gg 将其生物转化为香草酸，然后用 发酵生成香草醛。在孵育的第三天，通过 HPLC 检测到的香草酸的含量为 0.773g/L，而在随后的第三天孵育中香草醛的最佳产量为 0.628g/L。因此，通过从椰糠纤维中化学提取阿魏酸，然后进行生物转化为香草醛。这些产品具有很高的价值，在制药、健康、化妆品和营养保健品等行业具有很高的经济价值。
