Szczepańska Ewa, Pietrzak Witold, Boratyński Filip
Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland.
Department of Fermentation and Cereals Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland.
Molecules. 2025 Aug 22;30(17):3452. doi: 10.3390/molecules30173452.
Vanillin is the compound of great interest to the industry. It is used to augment and enhance the aroma and taste of food preparations and also as a fragrance compound in perfumes and detergents. Currently, majority of the world's supply consists of chemically synthesized or lignin-derived vanillin. The application of biocatalysis for sustainable manufacturing of food ingredients, pharmaceutical intermediates, and fine chemicals is the key concept of modern industrial biotechnology. The main goal of this research was to conduct optimization procedures aimed at intensifying the microbial hydrolysis process of the lignin-rich plant raw materials and further bioconversion of the released ferulic acid to vanillin. The tests were performed in the solid-state fermentation system with strains selected during the screening stage on agri-food by-products such as brewer's spent grain. A specially designed single-use bag bioreactor was used to carry out the process on a preparative scale with the most effective strain. The experiment was designed using the RSM, which allowed for an increase in biosynthesis efficiency from 363 mg/kg to 1413 mg/kg (an increase of 389%). The progress of the process was controlled by the use of chromatographic techniques (HPLC) by quantitative determination of vanillin content in the obtained extracts.
香草醛是该行业极具吸引力的化合物。它用于增强和提升食品制剂的香气和味道，还用作香水和洗涤剂中的香料化合物。目前，全球大部分供应的香草醛是化学合成的或源自木质素的。生物催化在食品成分、药物中间体和精细化学品可持续生产中的应用是现代工业生物技术的关键概念。本研究的主要目标是进行优化程序，以强化富含木质素的植物原料的微生物水解过程，并将释放的阿魏酸进一步生物转化为香草醛。试验在固态发酵系统中进行，使用在筛选阶段从诸如啤酒糟等农业食品副产品中挑选出的菌株。使用专门设计的一次性袋式生物反应器，以最有效的菌株在制备规模上进行该过程。实验采用响应面法设计，这使得生物合成效率从363毫克/千克提高到1413毫克/千克（提高了389%）。通过使用色谱技术（高效液相色谱法）对所得提取物中香草醛含量进行定量测定来控制该过程的进展。
