Departments of Periodontology and Implant Dentistry.
Oral and Maxillofacial Surgery.
Appl Immunohistochem Mol Morphol. 2021;29(5):e39-e45. doi: 10.1097/PAI.0000000000000880.
Recent evidence suggests that bone marrow-derived stem cells may have an important role in the natural process of wound healing. The aim of the present study was to examine the effect of orabase paste application on primary wound healing in a palatal rat model.
A total of 48 six-month-old male Wistar rats weighting 427 to 650 g were included. A mid-crestal incision was made on the maxillary alveolar ridge. A full-thickness flap was raised on either side of the incision and was then repositioned and sutured. Three experimental groups were used: O-study group-orabase, N-negative control group, and I-intact control group. Half of the animals were killed on 7 days and the remaining on 14 days postoperatively. Outcome parameters included epithelial gap; inflammatory infiltration; expression of stem cell markers within the oral epithelium and stromal cells; and physical properties of stromal collagen fibers and myofibroblasts. Investigations were performed at 2 time points (7 and 14 d) during the wound healing process.
The epithelial gap closed completely after 7 days in the O group versus 14 days or more in the N group. The inflammatory reaction was relatively low and not significantly different between groups O and N. Orabase upregulated the expression of CK14, CK15, and epithelial SOX2. Connective tissue SOX2, CD34, and α-smooth muscle actin and physical properties of stromal collagen fibers were not influenced by the application of orabase.
Orabase promotes epithelial gap closure in a primary wound healing model in rats. The effect is exerted through promotion of epithelial differentiation from stem cells.
最近的证据表明，骨髓来源的干细胞可能在伤口愈合的自然过程中发挥重要作用。本研究旨在检查奥沙利铂糊剂应用对腭部大鼠模型原发性伤口愈合的影响。
共纳入 48 只 6 月龄雄性 Wistar 大鼠，体重 427 至 650g。在上颌牙槽嵴的中央做一个垂直切口。在切口两侧掀起全层皮瓣，然后重新定位并缝合。使用 3 个实验组：O-研究组-奥沙利铂、N-阴性对照组和 I-完整对照组。一半的动物在术后 7 天和 14 天处死。观察指标包括上皮间隙、炎症浸润、口腔上皮和间质细胞中干细胞标志物的表达以及间质胶原纤维和肌成纤维细胞的物理特性。在伤口愈合过程中，在 2 个时间点（7 和 14d）进行研究。
O 组的上皮间隙在 7 天内完全闭合，而 N 组则需要 14 天或更长时间。O 组和 N 组之间的炎症反应相对较低，无显著差异。奥沙利铂上调 CK14、CK15 和上皮 SOX2 的表达。奥沙利铂对结缔组织 SOX2、CD34、α-平滑肌肌动蛋白和间质胶原纤维的物理特性没有影响。
奥沙利铂促进大鼠原发性伤口愈合模型中的上皮间隙闭合。这种作用是通过促进干细胞向上皮细胞分化来实现的。
