Kavut Büşra Sümeyye, Talmaç Ahmet Cemil, Önalan Şükrü
Department of Periodontology, Faculty of Dentistry, Van YüzüncüYıl University, Van, Turkey.
Department of Periodontology, Faculty of Dentistry, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey.
Mol Biol Rep. 2024 Dec 20;52(1):70. doi: 10.1007/s11033-024-10166-8.
This study aimed to investigate the effects of andiz extract on wound healing and compare it with saline and chlorhexidine gluconate. Microbial DNA load was used to evaluate its antibacterial effects, and gene expression methods were used to assess its contribution to cytokine release and wound healing.
A standardized wound site was created with a 3 mm diameter punch on 32 male Wistar albino rats. The rats were divided into four groups: Control (n = 5), Saline (n = 9), Chlorhexidine gluconate (n = 9), and Andiz extract (n = 9). Five rats in the control group were euthanised without any treatment. Irrigations of the Saline, Chlorhexidine, and Extract groups were provided regularly. After the tissue samples were taken in the 1st week, 2nd week, and 3rd week, three rats were euthanized each week for each group. The total bacterial DNA load on the samples taken was determined by a nano spectrophotometer. β-actin was chosen as housekeeping, and target gene primers were created for TGF-β and IL-1β. Expression amounts of target genes were measured by Real-Time PCR with the application of the created primers. There is a significant difference between the Extract group and the other groups regarding total bacterial DNA load. The whole bacterial load was 185% less than the initial values. TGF-β and IL-1β genes evaluated regarding gene expression were measured at the highest value in the Extract group.
This study showed the antibacterial effects of the Extract and its positive contributions to wound healing.
本研究旨在探讨安迪兹提取物对伤口愈合的影响，并将其与生理盐水和葡萄糖酸氯己定进行比较。采用微生物DNA负荷评估其抗菌效果，运用基因表达方法评估其对细胞因子释放和伤口愈合的作用。
在32只雄性Wistar白化大鼠身上，用直径3毫米的打孔器创建标准化伤口部位。将大鼠分为四组：对照组（n = 5）、生理盐水组（n = 9）、葡萄糖酸氯己定组（n = 9）和安迪兹提取物组（n = 9）。对照组的5只大鼠未经任何处理即实施安乐死。定期对生理盐水组、葡萄糖酸氯己定组和提取物组进行冲洗。在第1周、第2周和第3周采集组织样本后，每组每周对3只大鼠实施安乐死。用纳米分光光度计测定所采集样本上的总细菌DNA负荷。选择β-肌动蛋白作为管家基因，并针对转化生长因子-β（TGF-β）和白细胞介素-1β（IL-1β）设计靶基因引物。使用所设计的引物通过实时聚合酶链反应（Real-Time PCR）测量靶基因的表达量。提取物组与其他组在总细菌DNA负荷方面存在显著差异。总细菌负荷比初始值减少了185%。在提取物组中，就基因表达评估的TGF-β和IL-1β基因测量值最高。
本研究显示了提取物的抗菌效果及其对伤口愈合的积极作用。
