Humanitas Clinical and Research Center-IRCCS, Milan, Italy.
Department of Biomedical Sciences, Humanitas University, Milan, Italy.
Nat Immunol. 2021 Jan;22(1):19-24. doi: 10.1038/s41590-020-00832-x. Epub 2020 Nov 18.
Long pentraxin 3 (PTX3) is an essential component of humoral innate immunity, involved in resistance to selected pathogens and in the regulation of inflammation. The present study was designed to assess the presence and significance of PTX3 in Coronavirus Disease 2019 (COVID-19). RNA-sequencing analysis of peripheral blood mononuclear cells, single-cell bioinformatics analysis and immunohistochemistry of lung autopsy samples revealed that myelomonocytic cells and endothelial cells express high levels of PTX3 in patients with COVID-19. Increased plasma concentrations of PTX3 were detected in 96 patients with COVID-19. PTX3 emerged as a strong independent predictor of 28-d mortality in multivariable analysis, better than conventional markers of inflammation, in hospitalized patients with COVID-19. The prognostic significance of PTX3 abundance for mortality was confirmed in a second independent cohort (54 patients). Thus, circulating and lung myelomonocytic cells and endothelial cells are a major source of PTX3, and PTX3 plasma concentration can serve as an independent strong prognostic indicator of short-term mortality in COVID-19.
长型五聚素 3（PTX3）是体液先天免疫的重要组成部分，参与抵抗特定病原体和炎症调节。本研究旨在评估五聚素 3（PTX3）在 2019 年冠状病毒病（COVID-19）中的存在和意义。对外周血单核细胞进行 RNA 测序分析、单细胞生物信息学分析和肺尸检样本免疫组织化学分析表明，COVID-19 患者的髓样细胞和内皮细胞表达高水平的 PTX3。在 96 例 COVID-19 患者中检测到血浆 PTX3 浓度增加。在多变量分析中，PTX3 是 28 天死亡率的强独立预测因子，优于 COVID-19 住院患者的常规炎症标志物。PTX3 丰度对死亡率的预后意义在第二个独立队列（54 例患者）中得到证实。因此，循环和肺髓样细胞和内皮细胞是 PTX3 的主要来源，PTX3 血浆浓度可作为 COVID-19 短期死亡率的独立强预后指标。
